Binzunga Mamy Makumbu, Kokou Kintche, Mouritala Sikirou, Najimu Adetoro, Ibnou Dieng, Angelique Kajibwami, Mignouna Jacob, Nyende Aggrey Bernard
International Institute of Tropical Agriculture, Bukavu-Kalambo, Democratic Republic of the Congo.
Institut National pour l'Etude et la Recherche Agronomiques, Kinshasa, Democratic Republic of the Congo.
PLoS One. 2024 Dec 26;19(12):e0311437. doi: 10.1371/journal.pone.0311437. eCollection 2024.
The expansion of Semi-Autotrophic Hydroponics technology to address the issue of multiplying and disseminating virus-free planting materials for vegetatively propagated crops is challenged by the utilization of imported substrate, namely, KlasmannTS3. In this study, we evaluated the growth parameters and cutting production of cassava genotypes during three subsequent plantlet production cycles using three single substrates, namely, KlasmannTS3 (K), vermiculite (V), and local peat (P), and three blended substrates. The blended substrates were a combination of 25% K and 75% P (K25P75), a combination of V and P at respective rates of 25% and 75% (V25P75), and respective rates of 10% and 90% (V10P90). All cuttings obtained in one plantlet production cycle were transplanted into the next. The multiplication rate of cutting from cycle 1 to 2 (R1) and cycle 2 to 3 (R2) was calculated as the ratios of the number of cuttings per the number of plantlets in each cycle. K and K25P75 led to similar R1 and R2, except with the genotype IBA961089A, where K25P75 led to a higher R1. Local peat and V solely showed similar cutting multiplication rates, and were lower than V25P75 and V10P90. Substrates with a higher cutting production also led to a higher plantlet height, leaf, and internode number. V and its combinations with local peat led to the densest plantlet root system. The performance of the substrates contrasted among the genotypes, but IBA961089A mostly outperformed the two other genotypes. We concluded that up to 75% of K and, to a lesser extent 75% of V, can be substituted by P without compromising cutting production. V and P should be combined instead of being used separately.
半自养水培技术的扩展旨在解决无性繁殖作物无病毒种植材料的繁殖和传播问题，但该技术面临着进口基质（即克拉斯曼TS3）使用方面的挑战。在本研究中，我们评估了木薯基因型在三个连续的幼苗生产周期中的生长参数和插条产量，使用了三种单一基质，即克拉斯曼TS3（K）、蛭石（V）和当地泥炭（P），以及三种混合基质。混合基质分别是25%K和75%P的组合（K25P75）、V和P分别以25%和75%的比例组合（V25P75）以及10%和90%的比例组合（V10P90）。在一个幼苗生产周期中获得的所有插条都被移植到下一个周期。计算从第1周期到第2周期（R1）和第2周期到第3周期（R2）的插条繁殖率，即每个周期中插条数量与幼苗数量的比值。K和K25P75导致相似的R1和R2，但基因型IBA961089A除外，在该基因型中K25P75导致更高的R1。单独的当地泥炭和V表现出相似的插条繁殖率，且低于V25P75和V10P90。插条产量较高的基质也导致更高的幼苗高度、叶片数量和节间数量。V及其与当地泥炭的组合导致幼苗根系最密集。不同基因型之间基质的表现存在差异，但IBA961089A大多优于其他两种基因型。我们得出结论，高达75%的K以及在较小程度上75%的V可以被P替代，而不会影响插条产量。V和P应该组合使用而不是单独使用。
