Eskew M L, Scholz R W, Reddy C C, Todhunter D A, Zarkower A
Immunology. 1985 Jan;54(1):173-80.
The effects of dietary restriction of vitamin E and selenium were studied in male Long-Evans hooded rats. Weanling animals were maintained for 5-6 weeks on torula yeast-based diets, with or without the addition of vitamin E (150 IU/kg) or selenium (0.5 mg/kg), to form the following dietary groups: +E, +Se; +E, -Se; -E, +Se; -E, -Se, and a fifth group pair-fed with the -E, -Se group. This latter group exhibited a decreased rate of growth similar to the -E, -Se group. Lymphocyte blastogenesis in response to mitogens was decreased in animals fed the diets deficient in either vitamin E or selenium, and also in the pair-fed group. Very marked suppression of mitogen responses was seen in the doubly deficient group, as well as a greater loss of viability during culture. Spleen cell-mediated antibody-dependent lysis of chicken erythrocytes was increased in the doubly deficient group, although this difference could be abolished by the addition of catalase, but not indomethacin, to the culture medium. Dietary deficiency of vitamin E and selenium had no discernible effects on alveolar macrophage function, as measured by cell-mediated antibody-dependent cytolysis, killing of Staphylococcus aureus or regulation of T-lymphocyte blastogenesis.
在雄性长-伊斯特曼有帽大鼠中研究了维生素E和硒的饮食限制效果。将断奶动物在以产朊假丝酵母为基础的饮食上饲养5 - 6周，添加或不添加维生素E（150国际单位/千克）或硒（0.5毫克/千克），以形成以下饮食组：+E，+Se；+E，-Se；-E，+Se；-E，-Se，以及与-E，-Se组配对喂养的第五组。后一组的生长速率下降，与-E，-Se组相似。在喂食缺乏维生素E或硒的饮食的动物以及配对喂养组中，对有丝分裂原的淋巴细胞增殖反应降低。在双重缺乏组中观察到对有丝分裂原反应的非常明显的抑制，以及培养过程中更大的活力丧失。双重缺乏组中脾细胞介导的鸡红细胞抗体依赖性裂解增加，尽管通过在培养基中添加过氧化氢酶而不是消炎痛可以消除这种差异。通过细胞介导的抗体依赖性细胞溶解、金黄色葡萄球菌杀伤或T淋巴细胞增殖调节来测量，维生素E和硒的饮食缺乏对肺泡巨噬细胞功能没有明显影响。
