Chang W P, Hom J S, Dietert R R, Combs G F, Marsh J A
Department of Microbiology, Immunology, and Parasitology, Cornell University, Ithaca, NY 14853.
Immunopharmacol Immunotoxicol. 1994 May;16(2):203-23. doi: 10.3109/08923979409007091.
Beginning at hatching, chicks were fed a Basal diet, without vitamin E or selenium (Se) or the same diet supplemented with vitamin E (100 IU/kg) and Se (0.2 ppm). The effect of these treatments on the expression of cell surface markers (CT-1a, CD3, CD4, CD8, sIgs, and Ia) defining specific thymocyte and peripheral blood leukocyte (PBL) subpopulations were examined using flow cytometric analyses. In parallel studies the effect of the dietary deficiencies on splenocyte proliferative responses to ConA or PHA stimulation was examined. The mean expression of CD3 and CT-1a per cell was increased while CD8 and CD4 expression was decreased on thymocytes from chicks fed the Basal diet. The proportion of double negative (CD4-, CD8-) thymocytes and single positive CD8+ thymocytes was significantly decreased while single positive CD4+ and double positive (CD4+, CD8+) thymocytes were significantly increased by the dietary vitamin E and Se deficiencies. The dietary deficiencies resulted in a decreased proportion of peripheral T cells and specifically decreased the number of CD4+ PBL. The proliferative response to both ConA and PHA was impaired by the vitamin E and Se dietary deficiencies. The proliferative response could be fully reconstituted but only after vitamin E and Se supplementation for periods longer than 1 week. Plasma SeGSHpx and alpha-tocopherol levels paralleled the mitogen responsiveness observed. These results support the conclusion that vitamin E and Se deficiencies may affect both the maturation of specific lymphocyte subpopulations and the functional and proliferative capabilities of the peripheral lymphocytes.
从孵化开始，雏鸡被喂食基础日粮，该日粮不含维生素E或硒（Se），或者是添加了维生素E（100 IU/kg）和硒（0.2 ppm）的相同日粮。使用流式细胞术分析来检测这些处理对定义特定胸腺细胞和外周血白细胞（PBL）亚群的细胞表面标志物（CT-1a、CD3、CD4、CD8、sIgs和Ia）表达的影响。在平行研究中，检测了日粮缺乏对脾细胞对ConA或PHA刺激的增殖反应的影响。喂食基础日粮的雏鸡胸腺细胞上，每个细胞的CD3和CT-1a平均表达增加，而CD8和CD4表达降低。日粮中维生素E和硒缺乏导致双阴性（CD4-、CD8-）胸腺细胞和单阳性CD8+胸腺细胞的比例显著降低，而单阳性CD4+和双阳性（CD4+、CD8+）胸腺细胞显著增加。日粮缺乏导致外周T细胞比例降低，特别是CD4+ PBL数量减少。日粮中维生素E和硒缺乏损害了对ConA和PHA的增殖反应。增殖反应可以完全恢复，但仅在补充维生素E和硒超过1周后。血浆硒谷胱甘肽过氧化物酶（SeGSHpx）和α-生育酚水平与观察到的丝裂原反应性平行。这些结果支持以下结论：维生素E和硒缺乏可能影响特定淋巴细胞亚群的成熟以及外周淋巴细胞的功能和增殖能力。
