Langweiler M, Schultz R D, Sheffy B E
Am J Vet Res. 1981 Oct;42(10):1681-5.
The effect of vitamin E deficiency on the proliferative response of canine lymphocytes was examined, using the lymphocyte blastogenesis assay, with phytohemagglutinin, concanavalin A, and pokeweed as mitogenic stimulants. A litter (4 pups; group 3) from a bitch fed a complete diet was given a complete diet from the time of weaning (6 weeks) until termination of the experiment, and served as a control group. A litter (4 pups; group 1) from a bitch fed a vitamin E-deficient diet was given a vitamin E-deficient diet from the time of weaning (6 weeks) until termination of the experiment. A litter (6 pups; group 2) from a bitch fed a complete diet was weaned onto a complete diet and then changed to a deficient diet until termination of the experiment. Lymphocytes from group 1 and 2 pups (fed deficient diets) were poorly responsive to mitogen-induced blastogenesis, compared with the lymphocytic response seen in the controls. Washing the lymphocytes from the pups fed vitamin E-deficient diet resulted in enhanced responsiveness to mitogen stimulation. Lymphocytes from controls which had good responses when cultured in bovine fetal serum or normal canine serum were poorly responsive when cultured in serum from groups 1 and 2 pups (fed vitamin E-deficient diet). Reducing the concentration of group 1 and 2 pup sera in the culture medium restored the responsiveness of lymphocytes from control pups. Supplementation of group 1 and 2 pup sera with bovine fetal serum did not result in enhancement of the responses of lymphocytes from controls. The capability of sera from vitamin E-deficient pups (groups 1 and 2) to support blastogenesis of lymphocytes from control dogs was restored following dietary supplementation with vitamin E. It was concluded that the depressed lymphocyte responsiveness in pups fed vitamin E-deficient diet was due to the presense of a suppressive factor in the serum.
利用淋巴细胞增殖试验，以植物血凝素、刀豆球蛋白A和商陆作为促有丝分裂刺激剂，研究了维生素E缺乏对犬淋巴细胞增殖反应的影响。一窝（4只幼犬；第3组）来自喂食全价日粮母犬的幼犬，从断奶（6周龄）时起直至实验结束一直喂食全价日粮，作为对照组。一窝（4只幼犬；第1组）来自喂食维生素E缺乏日粮母犬的幼犬，从断奶（6周龄）时起直至实验结束一直喂食维生素E缺乏日粮。一窝（6只幼犬；第2组）来自喂食全价日粮母犬的幼犬，断奶后喂食全价日粮，然后改为缺乏日粮直至实验结束。与对照组淋巴细胞反应相比，第1组和第2组（喂食缺乏日粮）幼犬的淋巴细胞对有丝分裂原诱导的增殖反应较差。洗涤来自喂食维生素E缺乏日粮幼犬的淋巴细胞可增强其对有丝分裂原刺激的反应性。对照组的淋巴细胞在牛胎血清或正常犬血清中培养时反应良好，但在第1组和第2组（喂食维生素E缺乏日粮）幼犬的血清中培养时反应较差。降低培养基中第1组和第2组幼犬血清的浓度可恢复对照组幼犬淋巴细胞的反应性。向第1组和第2组幼犬血清中添加牛胎血清并未增强对照组淋巴细胞的反应。在日粮中补充维生素E后，维生素E缺乏幼犬（第1组和第2组）血清支持对照犬淋巴细胞增殖的能力得以恢复。得出的结论是，喂食维生素E缺乏日粮幼犬淋巴细胞反应性降低是由于血清中存在抑制因子。
