Curatolo L, Alessio G, Gambacorti Passerini C, Casali B, Morasca L, Semeraro N, Donati M B
Int J Cancer. 1985 Mar 15;35(3):411-4. doi: 10.1002/ijc.2910350318.
The presence of fibrin deposits in the microenvironment of tumor cells has been reported repeatedly and considered to play an important role in tumor biology. Among the mechanisms by which fibrin may be deposited in tumors, procoagulant activities (PCA) of different types have been described in cancer cells. The present study was aimed at establishing whether the nature of cellular PCA was a characteristic associated with malignant transformation. PCA of normal and transformed cells was investigated on pairs of murine and human origin. The transformed counterparts were obtained after treatment with low-dose radiation, chemical carcinogen, viral infection or after in vitro spontaneous immortalization. Both before and after any type of transformation cell PCA was of the tissue thromboplastin type, identified on the basis of biological criteria: requirement of factor VII for its expression and lack of inhibition by the serine protease inhibitor diisopropylfluorophosphate (DFP). Transformed cells of murine origin showed significantly lower activity than their normal counterparts, whereas all the transformed human cell lines expressed significantly higher activity than normal. An inverse correlation between the levels of PCA and the cell density in culture was observed in all but one of the lines tested. These findings suggest that the factor X activating property described in some tumors or in transformed cells cannot be considered as a general marker of transformation.
肿瘤细胞微环境中纤维蛋白沉积物的存在已被多次报道，并被认为在肿瘤生物学中发挥重要作用。在纤维蛋白可能沉积于肿瘤的机制中，癌细胞中已描述了不同类型的促凝血活性（PCA）。本研究旨在确定细胞PCA的性质是否是与恶性转化相关的特征。对源自小鼠和人类的成对正常细胞和转化细胞的PCA进行了研究。转化细胞是在低剂量辐射、化学致癌物、病毒感染处理后或体外自发永生化后获得的。在任何类型的转化之前和之后，细胞PCA均为组织凝血活酶类型，这是根据生物学标准确定的：其表达需要因子VII，且不受丝氨酸蛋白酶抑制剂二异丙基氟磷酸酯（DFP）的抑制。源自小鼠的转化细胞显示出比其正常对应细胞显著更低的活性，而所有转化的人类细胞系表达的活性均显著高于正常细胞。在所有测试的细胞系中，除一个细胞系外，均观察到PCA水平与培养物中细胞密度之间呈负相关。这些发现表明，在某些肿瘤或转化细胞中描述的因子X激活特性不能被视为转化的一般标志物。
