Lapoujade C, Blanco M, Givelet M, Gille A S, Allemand I, Lenez L, Thiounn N, Roux S, Wolf J P, Patrat C, Riou L, Barraud-Lange V, Fouchet P
Université Paris-Saclay, CEA, UMR Stabilité Génétique Cellules Souches Et Radiations, iRCM/IBFJ, Laboratoire Des Cellules Souches Germinales, 92265, Fontenay-Aux-Roses, France.
Université Paris Cité, CEA, UMR Stabilité Génétique Cellules Souches Et Radiations, iRCM/IBFJ, Laboratoire Des Cellules Souches Germinales, 92265, Fontenay-Aux-Roses, France.
Cell Mol Life Sci. 2024 Dec 26;82(1):15. doi: 10.1007/s00018-024-05496-6.
About one in six couples experience fertility problems, and male infertility accounts for about half of these cases. Spermatogenesis originates from a small pool of spermatogonial stem cells (SSCs), which are of interest for the treatment of infertility but remain poorly characterised in humans. Using multiparametric spectral flow cytometric analysis with a 16-colours (16-C) panel of cell markers, we identify novel markers of SSCs and provide insights into unravelling and resolving the heterogeneity of the human spermatogonial cells. This 16-C panel of markers allowed the identification of a primitive SSCs state with the β2MCD51/61ITGA6SSEA4TSPAN33THY1CD9EPCAMCD155CD148CD47CD7 phenotype, with a profile close to the most primitive SSCs states 0 and SSC1-B previously defined by sc-RNAseq approach. The hierarchy of events in the spermatogonial stem cell and progenitor compartment of human spermatogenesis can be delineated. This highlights the importance of a multi-parametric and spectral cytometry approach. The in-depth characterisation of testicular cells should help to overcome the lack of stem cell knowledge, that hinders the understanding of the regenerative potential of SSCs, and is a critical parameter for the successful development of new SSCs-based cell therapies.
约六分之一的夫妇存在生育问题，其中男性不育约占半数。精子发生源于一小群精原干细胞（SSCs），这些细胞对不育症治疗具有重要意义，但在人类中其特征仍知之甚少。通过使用具有16种细胞标志物的多参数光谱流式细胞术分析，我们鉴定出了精原干细胞的新标志物，并为揭示和解决人类精原细胞的异质性提供了见解。这一包含16种标志物的组合能够鉴定出具有β2MCD51/61ITGA6SSEA4TSPAN33THY1CD9EPCAMCD155CD148CD47CD7表型的原始精原干细胞状态，其特征与先前通过单细胞RNA测序方法定义的最原始精原干细胞状态0和精原干细胞1-B相近。人类精子发生过程中精原干细胞和祖细胞区室的事件层级得以描绘。这凸显了多参数光谱流式细胞术方法的重要性。对睾丸细胞的深入表征应有助于克服干细胞知识的不足，这种不足阻碍了对精原干细胞再生潜力的理解，而这是基于精原干细胞的新型细胞疗法成功开发的关键参数。
