Hickl Joachim, Argyropoulou Aikaterini, Al-Ahmad Ali, Hellwig Elmar, Skaltsounis Alexios Leandros, Wittmer Annette, Vach Kirstin, Karygianni Lamprini
Department of Operative Dentistry and Periodontology, Medical Center, Faculty of Medicine, University of Freiburg, Freiburg, Germany.
Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, National and Kapodistrian University of Athens, Athens, Greece.
Front Oral Health. 2024 Dec 12;5:1469174. doi: 10.3389/froh.2024.1469174. eCollection 2024.
The increasing demand for alternatives to antibiotics against resistant bacteria has led to research on natural products. The aim of this study was to analyze the antimicrobial and antibiofilm activity of 16 Mediterranean herb extracts.
The extracts were analyzed using High Performance Thin Layer Chromatography. The minimum inhibitory concentration and minimum bactericidal concentration of the extracts from , ssp. , , , , , , , , , , , , , , and were determined against eight oral bacteria and fungus . Microtiter plate test was conducted to evaluate the antibiofilm activity against .
Overall, all tested extracts efficiently suppressed the growth of obligate anaerobic bacteria. When applied at concentrations ≥0.15 mg/ml, the extracts exhibited moderate to high antibiofilm activity comparable to that of chlorhexidine (CHX) against . Interestingly, (MIC: 0.01-0.06 mg/ml) and (MIC: 0.04-1.25 mg/ml) demonstrated the highest antibacterial activity against oral bacteria. Additionally, and significantly inhibited biofilm formation at 0.15 mg/ml.
The tested plant extracts can be considered as alternative natural antimicrobial and antibiofilm agents.
Mediterranean herb extracts show promise as natural alternatives to combat oral bacteria and biofilm formation, offering potential new therapies for infectious oral diseases in the context of antibiotic resistance.
对抗生素耐药细菌的替代物需求不断增加，这促使人们对天然产物进行研究。本研究的目的是分析16种地中海草药提取物的抗菌和抗生物膜活性。
采用高效薄层色谱法对提取物进行分析。测定了来自[具体植物名称]、[具体植物名称]、[具体植物名称]、[具体植物名称]、[具体植物名称]、[具体植物名称]、[具体植物名称]、[具体植物名称]、[具体植物名称]、[具体植物名称]、[具体植物名称]、[具体植物名称]、[具体植物名称]、[具体植物名称]和[具体植物名称]的提取物对8种口腔细菌和真菌[具体真菌名称]的最低抑菌浓度和最低杀菌浓度。进行微量滴定板试验以评估对[具体细菌名称]的抗生物膜活性。
总体而言，所有测试提取物均能有效抑制专性厌氧菌的生长。当浓度≥0.15 mg/ml时，提取物表现出中度至高抗生物膜活性，与洗必泰（CHX）对[具体细菌名称]的活性相当。有趣的是，[具体植物名称]（MIC：0.01 - 0.06 mg/ml）和[具体植物名称]（MIC：0.04 - 1.25 mg/ml）对口腔细菌表现出最高的抗菌活性。此外，[具体植物名称]和[具体植物名称]在0.15 mg/ml时显著抑制[具体细菌名称]生物膜形成。
所测试的植物提取物可被视为替代天然抗菌和抗生物膜剂。
地中海草药提取物有望成为对抗口腔细菌和生物膜形成的天然替代品，在抗生素耐药的背景下为感染性口腔疾病提供潜在的新疗法。
