Department of Pediatric Dentistry, Dental Faculty, Qom University of Medical Sciences, Qom, Iran.
Cellular and Molecular Research Center, Qom University of Medical Sciences, Qom, Iran.
J Med Life. 2022 Apr;15(4):526-532. doi: 10.25122/jml-2021-0189.
There is a special focus on using natural materials and herbal plants to prevent dental caries. Previous studies showed that some herbal plants have antimicrobial effects on oral pathogens. Thus we investigated the antimicrobial effects of three herbal extracts (, , and ) on the growth of , as the most important bacteria causing dental caries. First, plant methanolic extracts were prepared. Then, to evaluate the antimicrobial activity of the three herbal extracts, the agar well diffusion method and MIC were performed. The biofilm formation was carried out using a broth dilution method with 2% glucose-supplemented BHIS in sterile 96-well microplates. Serial dilutions (50, 25, 12.5, 6.25, 3.12 mg/ml) of extracts were prepared. Next, a 0.5 McFarland Suspension of was added to wells. The inhibitory effect on biofilm formation was measured by the ELISA reader apparatus. The assay was repeated three times, and the average was calculated as 3. The results were compared with those of Chlorhexidine 0.2%. showed a better effect in the agar well diffusion method than others. MIC of the extracts of Carum coptimum, , and were 3.12, 6.25, and 12.5 mg/ml, respectively. Overall, the highest activity belonged to extract. For the anti-biofilm effect, the OD values of and were significantly different from that of . Although all of the methanolic herbal extracts can inhibit growth and remove the biofilm, the effect of was better than and . Further studies are recommended to indicate how these extracts perform against the bacteria.
特别关注使用天然材料和草药植物来预防龋齿。先前的研究表明，一些草药植物对口腔病原体具有抗菌作用。因此，我们研究了三种草药提取物（Carum copticum、Curcuma longa 和 Zingiber officinale）对作为引起龋齿最重要细菌的生长的抗菌作用。首先，制备植物甲醇提取物。然后，通过琼脂孔扩散法和 MIC 评估三种草药提取物的抗菌活性。使用含 2%葡萄糖的 BHIS 肉汤稀释法在无菌 96 孔微量板中进行生物膜形成。制备提取物的 50、25、12.5、6.25、3.12 mg/ml 的系列稀释液。接下来，向孔中加入 0.5 McFarland 浓度的。通过 ELISA 读取器装置测量对生物膜形成的抑制作用。该测定重复进行三次，平均值为 3。将结果与洗必泰 0.2%的结果进行比较。在琼脂孔扩散法中，Carum copticum 显示出比其他提取物更好的效果。Carum copticum、Curcuma longa 和 Zingiber officinale 的提取物 MIC 分别为 3.12、6.25 和 12.5 mg/ml。总体而言，活性最高的是 Carum copticum 提取物。对于抗生物膜作用，和的 OD 值与差异显著。虽然所有的甲醇草药提取物都可以抑制生长并去除生物膜，但提取物的效果优于和。建议进行进一步的研究以表明这些提取物对细菌的作用如何。
