Figueiredo Luciene Cristina, Bueno-Silva Bruno, Denúncio Giovanna, Figueiredo Nathalia Freitas, Cruz Daniele Ferreira da, Shibli Jamil A, Borges Maria Helena R, Barão Valentim A R, Haim Doron, Asbi Thabet, Souza João Gabriel S
Dental Research Division, Guarulhos University, Guarulhos 07023-070, SP, Brazil.
Department of Biosciences, Piracicaba Dental School, Universidade Estadual de Campinas (UNICAMP), Piracicaba 13414-903, SP, Brazil.
Gels. 2024 Nov 27;10(12):772. doi: 10.3390/gels10120772.
Evidence has shown the clear positive effects of nature-based products on biofilm control and improved gingival health. However, most studies have used in vitro models, have tested single natural components, or have not evaluated proteomic changes after treatment. This double-blind, parallel, randomized, and controlled clinical trial evaluated the benefits of a nature-based gel in controlling gingival inflammation and its effects on the proteomic gingival crevicular fluid (GCF) profile. Gingivitis patients were distributed into the following groups: (1) nature-based gel containing propolis, aloe vera, green tea, cranberry, and calendula (n = 10); (2) control-conventional toothpaste (n = 10). GCF was collected and evaluated by means of liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). At 3 months, the groups showed similar clinical benefits ( < 0.05). A total of 480 proteins were identified across all groups. In a pooled comparison of both groups at both time points, exclusive proteins were identified in the nature-based gel (78) and the control (21) groups. The exclusive proteins identified for the toothpaste mainly acted in wound healing, and those for the nature-based gel mainly acted on immune system processes. The nature-based gel achieved similar clinical outcomes to conventional toothpaste. However, the nature-based gel markedly changed the proteomic profile of GCF after treatment, showing a profile associated with a host response.
有证据表明，天然产品对生物膜控制和改善牙龈健康具有明显的积极作用。然而，大多数研究使用的是体外模型，测试的是单一天然成分，或者没有评估治疗后的蛋白质组学变化。这项双盲、平行、随机对照临床试验评估了一种天然凝胶在控制牙龈炎症方面的益处及其对牙龈沟液（GCF）蛋白质组学特征的影响。牙龈炎患者被分为以下几组：（1）含有蜂胶、芦荟、绿茶、蔓越莓和金盏花的天然凝胶组（n = 10）；（2）对照-传统牙膏组（n = 10）。通过液相色谱-串联质谱联用（LC-MS/MS）收集并评估牙龈沟液。3个月时，两组显示出相似的临床益处（< 0.05）。所有组共鉴定出480种蛋白质。在两个时间点对两组进行的汇总比较中，在天然凝胶组（78种）和对照组（21种）中鉴定出了独特的蛋白质。牙膏组鉴定出的独特蛋白质主要作用于伤口愈合，而天然凝胶组的主要作用于免疫系统过程。天然凝胶取得了与传统牙膏相似的临床效果。然而，天然凝胶在治疗后显著改变了牙龈沟液的蛋白质组学特征，显示出与宿主反应相关的特征。
