Grupo de Biotecnología y Bioprocesos Marinos, Instituto de Investigaciones Marinas (CSIC), 36208 Vigo, Spain.
Laboratorio de Bioquímica de Alimentos, Instituto de Investigaciones Marinas (CSIC), 36208 Vigo, Spain.
Mar Drugs. 2019 Jan 9;17(1):40. doi: 10.3390/md17010040.
The small-spotted catshark is one of the most abundant elasmobranchs in the Northeastern Atlantic Ocean. Although its landings are devoted for human consumption, in general this species has low commercial value with high discard rates, reaching 100% in some European fisheries. The reduction of post-harvest losses (discards and by-products) by promotion of a full use of fishing captures is one of the main goals of EU fishing policies. As marine collagens are increasingly used as alternatives to mammalian collagens for cosmetics, tissue engineering, etc., fish skins represent an excellent and abundant source for obtaining this biomolecule. The aim of this study was to analyze the influence of chemical treatment concentration, temperature and time on the extractability of skin collagen from this species. Two experimental designs, one for each of the main stages of the process, were performed by means of Response Surface Methodology (RSM). The combined effect of NaOH concentration, time and temperature on the amount of collagen recovered in the first stage of the collagen extraction procedure was studied. Then, skins treated under optimal NaOH conditions were subjected to a second experimental design, to study the combined effect of AcOH concentration, time and temperature on the collagen recovery by means of yield, amino acid content and SDS-PAGE characterization. Values of independent variables maximizing collagen recovery were 4 °C, 2 h and 0.1 M NaOH (pre-treatment) and 25 °C, 34 h and 1 M AcOH (collagen extraction).
小斑竹鲨是东北大西洋最丰富的软骨鱼之一。尽管其捕捞上岸是供人类食用，但该物种的商业价值通常较低，丢弃率很高，在一些欧洲渔业中达到 100%。通过促进充分利用渔获物来减少收获后的损失（丢弃物和副产品）是欧盟渔业政策的主要目标之一。由于海洋胶原蛋白越来越多地被用作替代哺乳动物胶原蛋白用于化妆品、组织工程等，鱼类皮代表了获得这种生物分子的极好且丰富的来源。本研究的目的是分析化学处理浓度、温度和时间对该物种皮肤胶原蛋白提取率的影响。通过响应面法（RSM）进行了两个实验设计，分别针对该过程的主要阶段之一。研究了 NaOH 浓度、时间和温度对胶原蛋白提取过程第一阶段胶原蛋白回收量的综合影响。然后，对在最佳 NaOH 条件下处理的皮进行了第二次实验设计，以研究 AcOH 浓度、时间和温度对胶原蛋白回收率的综合影响，方法是通过产率、氨基酸含量和 SDS-PAGE 特性进行研究。使胶原蛋白回收率最大化的自变量值为 4°C、2 h 和 0.1 M NaOH（预处理）以及 25°C、34 h 和 1 M AcOH（胶原蛋白提取）。
