Al-Ishaq Raghad Khalid, Ferrara Carmen R, Stephan Nisha, Krumsiek Jan, Suhre Karsten, Montrose David C
Bioinformatics Core, Weill Cornell Medicine-Qatar, Cornell University, Education City, Doha 24144, Qatar.
Department of Pathology, Renaissance School of Medicine, Stony Brook University, MART Building, 9M-0816, Lauterbur Dr., Stony Brook, NY 11794, USA.
Metabolites. 2024 Dec 14;14(12):706. doi: 10.3390/metabo14120706.
Nutritional metabolomics provides a comprehensive overview of the biochemical processes that are induced by dietary intake through the measurement of metabolite profiles in biological samples. However, there is a lack of deep phenotypic analysis that shows how dietary interventions influence the metabolic state across multiple physiologic sites. Dietary amino acids have emerged as important nutrients for physiology and pathophysiology given their ability to impact cell metabolism. The aim of the current study is to evaluate the effect of modulating amino acids in diet on the metabolome and microbiome of mice. Here, we report a comprehensive metabolite profiling across serum, liver, and feces, in addition to gut microbial analyses, following a reduction in either total dietary protein or diet-derived non-essential amino acids in mice. We observed both distinct and overlapping patterns in the metabolic profile changes across the three sample types, with the strongest signals observed in liver and serum. Although amino acids and related molecules were the most commonly and strongly altered group of metabolites, additional small molecule changes included those related to glycolysis and the tricarboxylic acid cycle. Microbial profiling of feces showed significant differences in the abundance of select species across groups of mice. Our results demonstrate how changes in dietary amino acids influence the metabolic profiles across organ systems and the utility of metabolomic profiling for assessing diet-induced alterations in metabolism.
营养代谢组学通过测量生物样本中的代谢物谱，全面概述了饮食摄入所引发的生化过程。然而，目前缺乏深入的表型分析来展示饮食干预如何影响多个生理部位的代谢状态。鉴于膳食氨基酸能够影响细胞代谢，它们已成为生理学和病理生理学中的重要营养素。本研究的目的是评估调节饮食中的氨基酸对小鼠代谢组和微生物组的影响。在此，我们报告了在小鼠总膳食蛋白质或膳食来源的非必需氨基酸减少后，除肠道微生物分析外，血清、肝脏和粪便中的全面代谢物谱。我们在三种样本类型的代谢谱变化中观察到了不同和重叠的模式，其中在肝脏和血清中观察到的信号最强。尽管氨基酸和相关分子是最常且强烈改变的代谢物组，但其他小分子变化包括与糖酵解和三羧酸循环相关的变化。粪便的微生物谱显示，不同组小鼠中特定物种的丰度存在显著差异。我们的结果证明了膳食氨基酸的变化如何影响跨器官系统的代谢谱，以及代谢组学分析在评估饮食诱导的代谢变化中的作用。
