Zhao Yang, Liu Fang, Lan Xing, Xu Wenxing, Dong Wubei, Ke Shaoyong, Wu Hongqu
Key Laboratory of Microbial Pesticides (Ministry of Agriculture and Rural Affairs), National Biopesticide Engineering Research Centre, Hubei Biopesticide Engineering Research Centre, Hubei Academy of Agricultural Sciences, Wuhan 430064, China.
College of Plant Science and Technology, Huazhong Agricultural University, and Key Laboratory of Microbial Pesticides, Ministry of Agriculture and Rural Affairs, Wuhan 430070, China.
J Agric Food Chem. 2025 Feb 5;73(5):2793-2804. doi: 10.1021/acs.jafc.4c09294. Epub 2024 Dec 27.
This study investigates a novel antimicrobial peptide AtR905 derived from the endophytic fungus , which was successfully expressed in , purified, and characterized, and highlighted as a promising potential biocontrol agent against various plant pathogens. The results indicated AtR905 exhibited broad-spectrum antimicrobial activities against key pathogens such as and with very low minimal inhibitory concentrations (MICs). Stability tests confirmed that AtR905 retains its antimicrobial properties under varying thermal, pH, and UV conditions. The Oxford Cup test indicated that AtR905 showed obvious fungicidal activity against six plant pathogenic fungi, especially and . Additionally, in vivo experimental demonstrated AtR905 could effectively control the on tobacco leaves and on tomato plants. Scanning electron microscopy revealed significant membrane disruption in bacterial cells treated with AtR905. These findings suggest that AtR905 is a promising candidate for sustainable plant disease management, potentially reducing the reliance on chemical pesticides and mitigating the issue of antibiotic resistance in agricultural settings. Further research is needed to evaluate the long-term field applicability and ecological impacts of AtR905.
本研究调查了一种源自内生真菌的新型抗菌肽AtR905，该抗菌肽已成功表达、纯化并进行了表征，并被视为一种有前景的针对多种植物病原体的潜在生物防治剂。结果表明，AtR905对关键病原体如[具体病原体1]和[具体病原体2]表现出广谱抗菌活性，其最低抑菌浓度（MIC）非常低。稳定性测试证实，AtR905在不同的热、pH和紫外线条件下仍保留其抗菌特性。牛津杯试验表明，AtR905对六种植物病原真菌表现出明显的杀真菌活性，尤其是[具体真菌1]和[具体真菌2]。此外，体内实验表明AtR905可以有效控制烟草叶片上的[具体病害1]和番茄植株上的[具体病害2]。扫描电子显微镜显示，用AtR905处理的细菌细胞出现明显的膜破坏。这些发现表明，AtR905是可持续植物病害管理的一个有前景的候选物，有可能减少对化学农药的依赖，并缓解农业环境中的抗生素耐药性问题。需要进一步研究来评估AtR905的长期田间适用性和生态影响。
