一种无需培养即可对腺病毒40型和41型进行分型的简单快速方法。
A simple and rapid method for typing adenoviruses 40 and 41 without cultivation.
作者信息
Buitenwerf J, Louwerens J J, De Jong J C
出版信息
J Virol Methods. 1985 Jan;10(1):39-44. doi: 10.1016/0166-0934(85)90086-2.
PMID:3972942
Abstract
Fastidious adenovirus DNA in faecal samples obtained from children with acute gastroenteritis was extracted by a simple and rapid method. The extracted viral DNA was characterized by restriction endonuclease SmaI treatment. Fastidious adenovirus DNA was detected in 58 of 65 cases. If faecal samples were too small it was not always possible to identify virus DNA.
摘要
采用一种简单快速的方法从患有急性肠胃炎的儿童粪便样本中提取难培养腺病毒DNA。提取的病毒DNA通过限制性内切酶SmaI处理进行鉴定。65例样本中有58例检测到难培养腺病毒DNA。如果粪便样本量过少，并不总是能够鉴定出病毒DNA。
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