Vinijkumthorn Ruethaiwan, Kingkaw Amornthep, Yanyongsirikarn Petchpailin, Phaonakrop Narumon, Roytrakul Sittiruk, Vongsangnak Wanwipa, Tesena Parichart
Department of Clinical Science and Public Health, Faculty of Veterinary Science, Mahidol University, Salaya, Puttamonthon, Nakhon Pathom, 73170, Thailand.
Interdisciplinary Graduate Program in Bioscience, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand.
Sci Rep. 2024 Dec 28;14(1):30842. doi: 10.1038/s41598-024-81338-6.
Equine melanocytic neoplasm (EMN) represents a form of skin tumor observed predominantly in grey horses aged over 15 years. Despite its prevalence, current therapeutic and preventive strategies for EMN have been subject to limited investigation. This study endeavors to shed light on potential phosphoproteins present in equine serum and fecal samples, potentially linked to EMN, with a specific focus on functional interactions in EMN pathogenesis. We examined 50 samples (25 serum, 25 feces), divided into three groups based on EMN severity: normal (n = 16), mild (n = 18), and severe EMN (n = 16). Equine phosphoproteome analysis identified 2,359 annotated serum phosphoproteins and 2002 annotated fecal phosphoproteins through differentially expressed proteins (DEPs). KEGG analysis emphasized the role of environmental information processing. Notably, the integrin NF-kappaB binding P-TEFb to stimulate transcriptional elongation signaling pathway, involving SNW1 protein, was implicated in early stage of EMN development in both serum and fecal samples. This highlights SNW1's potential role in mediating transcriptional processes, offering a novel marker within environmental information processing. This study enhances understanding of EMN mechanisms in horses, suggesting early detection through non-invasive methods and identifying a functional pathway involving SNW1, which could inform future treatment and prevention strategies.
马黑素细胞瘤（EMN）是一种主要在15岁以上的灰色马匹中观察到的皮肤肿瘤形式。尽管其发病率较高，但目前针对EMN的治疗和预防策略的研究有限。本研究旨在揭示马血清和粪便样本中可能与EMN相关的潜在磷酸化蛋白，特别关注EMN发病机制中的功能相互作用。我们检查了50个样本（25份血清，25份粪便），根据EMN严重程度分为三组：正常（n = 16）、轻度（n = 18）和重度EMN（n = 16）。马磷酸化蛋白质组分析通过差异表达蛋白（DEP）鉴定出2359种注释的血清磷酸化蛋白和2002种注释的粪便磷酸化蛋白。KEGG分析强调了环境信息处理的作用。值得注意的是，整合素NF-κB结合P-TEFb以刺激转录延伸信号通路，涉及SNW1蛋白，在血清和粪便样本中均与EMN发展的早期阶段有关。这突出了SNW1在介导转录过程中的潜在作用，在环境信息处理中提供了一个新的标志物。本研究增进了对马EMN机制的理解，建议通过非侵入性方法进行早期检测，并确定了一条涉及SNW1的功能途径，这可为未来的治疗和预防策略提供参考。
