Zhang Wenhua, Xie Meng, Huang Qiang, Liu Honglei, Liu Jianfeng, Sun Xiaoli, Li Conghui
Department of Neurosurgery, The First Hospital of Hebei Medical University, Shijiazhuang, 050000, Hebei, China.
Department of Pediatrics, Shijiazhuang People,s Hospital, Shijiazhuang, 050000, Hebei, China.
Sci Rep. 2024 Dec 28;14(1):30994. doi: 10.1038/s41598-024-82145-9.
Six-transmembrane epithelial antigen of prostate 3 (STEAP3), a member of the iron regulation protein family, is characterized by a high recurrence rate and a short survival time. Nevertheless, studies investigating the role of STEAP3 in glioblastoma (GB) are scarce. In this study, the prognostic value of STEAP3 was evaluated utilizing mRNA expression profiles from Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) databases as the validation and training cohorts, respectively. Moreover, differentially expressed genes were subjected to a functional enrichment analysis. The relationship between STEAP3 and the tumor microenvironment (TME) was examined. The immunophenoscore (IPS) and tumor immune dysfunction and exclusion (TIDE) score were used to investigate response to immunotherapy. In all cohorts, GB patients with higher STEAP3 expression levels exhibited shorter overall survival (OS). Additionally, STEAP3-associated genes were primarily implicated in leukocyte migration, the JAK-STAT signaling pathway, and the cytokine-mediated signaling pathway. In the STEAP3 high-expression group, the ESTIMATEScore, ImmuneScore, StromalScore, and IPS were significantly higher. Overall, our results highlighted that STEAP3 might serve as a candidate prognostic biomarker for GB. Additionally, it might regulate the TME and influence GB metastasis.
前列腺六跨膜上皮抗原3（STEAP3）是铁调节蛋白家族的一员，其特点是复发率高、生存时间短。然而，关于STEAP3在胶质母细胞瘤（GB）中作用的研究很少。在本研究中，分别利用基因表达综合数据库（GEO）和癌症基因组图谱（TCGA）数据库中的mRNA表达谱作为验证队列和训练队列，评估STEAP3的预后价值。此外，对差异表达基因进行了功能富集分析。研究了STEAP3与肿瘤微环境（TME）之间的关系。使用免疫表型评分（IPS）和肿瘤免疫功能障碍与排除（TIDE）评分来研究对免疫治疗的反应。在所有队列中，STEAP3表达水平较高的GB患者总生存期（OS）较短。此外，与STEAP3相关的基因主要涉及白细胞迁移、JAK-STAT信号通路和细胞因子介导的信号通路。在STEAP3高表达组中，ESTIMATEScore、免疫评分、基质评分和IPS显著更高。总体而言，我们的结果表明，STEAP3可能作为GB的候选预后生物标志物。此外，它可能调节TME并影响GB转移。
