Zamani Elnaz, Bakhtari Bahlanes, Razi Hooman, Hildebrand David, Moghadam Ali, Alemzadeh Abbas
Department of Plant Production and Genetics, School of Agriculture, Shiraz University, Shiraz, Iran.
Department of Plant & Soil Sciences, University of Kentucky, Lexington, KY, 40546, USA.
Sci Rep. 2024 Dec 28;14(1):31036. doi: 10.1038/s41598-024-82173-5.
Salinity and lead are two important abiotic stresses that limit crop growth and yield. In this study, we assayed the effect of these stresses on tolerant and sensitive maize genotypes. Four-week-old maize plants were treated with 250 mM sodium chloride (NaCl) and 250 µM lead (Pb). Our results show that NaCl or Pb treatment of the sensitive genotype caused a significant reduction in the root length, plant height, total fresh and dry weights, as well as chlorophyll, and carotenoid content. Salt stress led to a significant decrease in the relative water content, shoot and root length, fresh and dry weight as well as leaf area and K content but increase Na content. Both NaCl and Pb stresses increased the antioxidant enzyme activity, proline content, malondialdehyde, and hydrogen peroxide levels. Principal component analysis (PCA) accounted for 69.8% and 16.5% of the total variation among all the variables studied. PCA also suggested a positive correlation between hydrogen peroxide, malondialdehyde, peroxidase, catalase, ascorbate peroxidase levels, and Na content and a negative correlation between K content, chlorophyll content, relative water content, leaf area, root length, plant height, and total fresh, and dry weights. Together, these results suggest that the salt-tolerant maize genotype is more suitable for adapting to Pb stress compared to the salt-sensitive genotype.
盐分和铅是限制作物生长和产量的两种重要非生物胁迫。在本研究中，我们测定了这些胁迫对耐性和敏感型玉米基因型的影响。对四周龄的玉米植株用250 mM氯化钠（NaCl）和250 µM铅（Pb）进行处理。我们的结果表明，对敏感基因型进行NaCl或Pb处理会导致根长、株高、总鲜重和干重以及叶绿素和类胡萝卜素含量显著降低。盐胁迫导致相对含水量、地上部和根长、鲜重和干重以及叶面积和钾含量显著下降，但钠含量增加。NaCl和Pb胁迫均增加了抗氧化酶活性、脯氨酸含量、丙二醛和过氧化氢水平。主成分分析（PCA）解释了所有研究变量中总变异的69.8%和16.5%。PCA还表明过氧化氢、丙二醛、过氧化物酶、过氧化氢酶、抗坏血酸过氧化物酶水平与钠含量之间呈正相关，而钾含量、叶绿素含量、相对含水量、叶面积、根长、株高以及总鲜重和干重之间呈负相关。总之，这些结果表明，与盐敏感基因型相比，耐盐玉米基因型更适合适应铅胁迫。
