Yongkai Wang, Shuhui Zhang, Li Ma
Department of Stomatology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, No.51, Weiliu Road, Jinan, Shandong Province, 250021, China.
Stomatological College, Shandong First Medical University, Jinan, Shandong Province, China.
J Mol Histol. 2024 Dec 28;56(1):60. doi: 10.1007/s10735-024-10331-0.
2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is an important environmental pollutant that disturbs the immune balance of the maternal-fetal interface (MFI) and is also a common environmental factor for the formation of cleft palate (CP). Therefore, the purpose is to investigate whether TCDD can cause CP by disrupting the immune balance of the maternal-fetal interface. Fifteen C57BL/6J mice were randomly assigned to three groups: control group, TCDD group, and TCDD plus Freund's complete adjuvant (FCA) (TCDD + FCA) group. Peripheral blood, placentas, and palatal tissues were collected for H&E, flow cytometry, and ELISA. In the TCDD group, the placental diameter, the number of placental labyrinth vessels, and the area of sponge layer cells were all significantly reduced. At embryonic day (E) 17.0, there was a significant decrease in T-helper 1 (Th1) and Th2 cells in the peripheral blood of pregnant mice. Additionally, the levels of interferon-γ (IFN-γ) and interleukin-4 (IL-4), particularly IL-4, were significantly decreased. However, after treatment with FCA, the distance between the palatal shelves was reduced, and the placental weight, the number of labyrinth vessels, and the area of the cavernous cells in the placenta also increased. The number of Th1 and Th2 cells significantly increased, returning to the levels observed in the control group, with a more pronounced increase in the number of Th2 cells. In conclusion, TCDD may induce CP by disrupting the homeostasis of the MFI. The precise mechanisms by which TCDD impacts the immune system at the MFI require further investigation.2,3,7,8-- （TCDD） ， （MFI） ， （CP） 。， TCDD CP。 15 C57BL/6J 3 ：、TCDD TCDD （FCA） （TCDD + FCA） 。、 H&E、 ELISA。TCDD 、。 17.0 （E） ， T 1 （Th1） Th2 。，-γ （IFN-γ） -4 （IL-4）， IL-4 。， FCA ，，、。Th1 Th2 ，，Th2 。，TCDD MFI CP。TCDD MFI 。.
2,3,7,8-四氯二苯并对二恶英（TCDD）是一种重要的环境污染物，它会扰乱母胎界面（MFI）的免疫平衡，也是腭裂（CP）形成的常见环境因素。因此，目的是研究TCDD是否会通过破坏母胎界面的免疫平衡而导致CP。将15只C57BL/6J小鼠随机分为三组：对照组、TCDD组和TCDD加弗氏完全佐剂（FCA）（TCDD+FCA）组。采集外周血、胎盘和腭组织进行苏木精-伊红染色（H&E）、流式细胞术和酶联免疫吸附测定（ELISA）。在TCDD组中，胎盘直径、胎盘迷路血管数量和海绵层细胞面积均显著减小。在胚胎第17.0天（E），妊娠小鼠外周血中的辅助性T细胞1（Th1）和Th2细胞显著减少。此外，γ干扰素（IFN-γ）和白细胞介素-4（IL-4）的水平，尤其是IL-4，显著降低。然而，用FCA处理后，腭突间距离减小，胎盘重量、迷路血管数量和胎盘海绵状细胞面积也增加。Th1和Th2细胞数量显著增加，恢复到对照组观察到的水平，其中Th2细胞数量增加更为明显。总之，TCDD可能通过破坏MFI的稳态而诱导CP。TCDD在MFI处影响免疫系统的确切机制需要进一步研究。
