Department of Infectious Diseases, First Affiliated Hospital, Guangxi Medical University, Nanning 530021, China.
Department of Physics, The University of Texas at Arlington, Arlington, TX 76019-0059, USA.
Mater Sci Eng C Mater Biol Appl. 2020 May;110:110659. doi: 10.1016/j.msec.2020.110659. Epub 2020 Jan 11.
Herein, for the first time, we demonstrate that the combination of copper-cysteamine (Cu-Cy) nanoparticles (NPs) and potassium iodide (KI) can significantly inactivate both Gram-positive MRSA and Gram-negative E. coli. To uncover the mystery of the killing, the interaction of KI with Cu-Cy NPs was investigated systematically and the products from their interaction were identified. No copper ions were released after adding KI to Cu-Cy NPs in cell-free medium and, therefore, it is reasonable to conclude that the Fenton reaction induced by copper ions is not responsible for the bacterial killing. Based on the observations, we propose that the major killing mechanism involves the generation of toxic species, such as hydrogen peroxide, triiodide ions, iodide ions, singlet oxygen, and iodine molecules. Overall, the powerful combination of Cu-Cy NPs and KI has good potential as an independent treatment or a complementary antibiotic treatment to infectious diseases.
在此，我们首次证明，铜-半胱氨酸（Cu-Cy）纳米颗粒（NPs）与碘化钾（KI）的组合可以显著灭活革兰氏阳性耐甲氧西林金黄色葡萄球菌（MRSA）和革兰氏阴性大肠杆菌（E. coli）。为了揭示杀菌的奥秘，我们系统地研究了 KI 与 Cu-Cy NPs 的相互作用，并鉴定了它们相互作用的产物。在无细胞介质中向 Cu-Cy NPs 中加入 KI 后没有释放出铜离子，因此可以合理地得出结论，铜离子诱导的芬顿反应不是导致细菌杀伤的原因。基于这些观察结果，我们提出主要的杀伤机制涉及有毒物质的产生，如过氧化氢、三碘化物离子、碘化物离子、单线态氧和碘分子。总的来说，Cu-Cy NPs 和 KI 的强大组合具有作为独立治疗或作为传染病辅助抗生素治疗的良好潜力。
