采用聚乙二醇沉淀结合酶联免疫吸附测定法检测循环免疫复合物。
Detection of circulating immune complexes by PEG precipitation combined with ELISA.
作者信息
Ohlson S, Zetterstrand K
出版信息
J Immunol Methods. 1985 Feb 28;77(1):87-93. doi: 10.1016/0022-1759(85)90186-3.
An assay to detect circulating immune complexes is described and exemplified with serum from SLE and RA patients. The method is based on selective precipitation of immune complexes with PEG followed by a specific ELISA assay to detect the immunoglobulins present in the precipitate. The immunoglobulins of the precipitate were then monitored by their capacity to bind protein A and dDNA. With the PEG-protein A-ELISA procedure immune complexes were detected in 46% of the sera from SLE patients compared with 68% when using the PEG-DNA-ELISA.
本文描述了一种检测循环免疫复合物的分析方法，并以系统性红斑狼疮（SLE）和类风湿关节炎（RA）患者的血清为例进行了说明。该方法基于用聚乙二醇（PEG）选择性沉淀免疫复合物，随后通过特异性酶联免疫吸附测定（ELISA）来检测沉淀物中存在的免疫球蛋白。然后通过沉淀物结合蛋白A和双链DNA（dDNA）的能力来监测其中的免疫球蛋白。采用PEG-蛋白A-ELISA方法时，在46%的SLE患者血清中检测到了免疫复合物，而使用PEG-DNA-ELISA时这一比例为68%。
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