Yuan Luohua, Liu Haibing, Li Wenli, Peng Zhonghua, Ma Yuling, Zou Jian
Department of Otolaryngology Head and Neck Surgery，West China Hospital，Sichuan University，Chengdu，610000，China.
Otolaryngology Department of Sichuan Maternal and Child Health Hospital.
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2025 Jan;39(1):51-56. doi: 10.13201/j.issn.2096-7993.2025.01.011.
To explore the hypothesis of "pathogen storage pool" by analyzing the local microbial community of adenoids. Under the guidance of a 70° nasal endoscope, sterile swabs were used to collect secretions from the adenoid crypts of the subjects. The samples were sent to the laboratory for DNA extraction and standard bacterial 16S full-length sequencing analysis. At the species level, the top three microbial communities in adenoid crypts were Bacillus subtilis（18.78%）, Fusobacterium pyogenes（11.42%）, and Streptococcus pneumoniae（9.38%）. The local microbial community of adenoids exhibits a high degree of diversity, including microbial communities from the oral cavity and gastrointestinal tract. Our research results support the hypothesis that adenoids act as a " pathogen reservoir".
通过分析腺样体局部微生物群落来探讨“病原体储存库”假说。在70°鼻内窥镜引导下，用无菌拭子收集受试者腺样体隐窝分泌物。样本送至实验室进行DNA提取和标准细菌16S全长测序分析。在物种水平上，腺样体隐窝中排名前三的微生物群落分别是枯草芽孢杆菌（18.78%）、化脓性梭杆菌（11.42%）和肺炎链球菌（9.38%）。腺样体局部微生物群落具有高度多样性，包括来自口腔和胃肠道的微生物群落。我们的研究结果支持腺样体充当“病原体储存库”这一假说。
