Zhao Lu, Fang Yongkun, Zhang Jingqiu, Wei Chen, Ji Hao, Zhao Jiahao, Wang Daorong, Tang Dong
The Yangzhou Clinical College of Xuzhou Medical University, Xuzhou Medical University, Yangzhou, China.
Northern Jiangsu People's Hospital, Yangzhou, China.
Clin Med Insights Oncol. 2024 Dec 24;18:11795549241307632. doi: 10.1177/11795549241307632. eCollection 2024.
Gut microbiota are associated with the pathological features and development of colorectal cancer (CRC); however, how gut microbiota changes in patients with CRC is unknown. This study investigated the role of gut microbiota in the development and progression of CRC by retrospectively comparing the structural differences between the gut microbiota of patients with CRC and healthy individuals.
Together with clinical data, we collected fecal samples from patients with CRC (n = 18) and healthy controls (n = 18) and performed 16S rRNA gene sequencing and alpha and beta diversity analysis to compare microbiota richness and diversity. Based on the differences in microbiota between the CRC and control groups, we identified disease-specific microbial communities after relevant factors. PICRUSt2 software was used to predict the differential microbial functions.
The CRC and control groups differed in both composition and abundance of intestinal microbiota. Firmicutes and Bacteroidetes were the most abundant phyla in both groups, while Verrucomicrobi was significantly more abundant in the CRC group. , , and were more abundant in the control group; 18 genera differed significantly in abundance between the groups, which were found to involve 21 metabolic pathways. The distribution and abundance of gut microbiota differed significantly between patients with CRC with and without lymph node metastasis; at the genus level, the abundance of and was significantly higher and that of , , and was significantly lower in patients with lymph node metastasis.
The gut microbiota is altered in CRC patients compared with healthy individuals, with specific changes in the microbiota associated with clinical and pathological features such as tumor stage, lymph node involvement, and tumor differentiation. Our findings elaborate to some extent on the link between the gut microbiota and CRC.
肠道微生物群与结直肠癌（CRC）的病理特征及发展相关；然而，CRC患者肠道微生物群如何变化尚不清楚。本研究通过回顾性比较CRC患者与健康个体肠道微生物群的结构差异，探讨肠道微生物群在CRC发生发展中的作用。
我们收集了CRC患者（n = 18）和健康对照者（n = 18）的粪便样本及临床数据，进行16S rRNA基因测序以及α和β多样性分析，以比较微生物群的丰富度和多样性。基于CRC组与对照组微生物群的差异，在考虑相关因素后确定疾病特异性微生物群落。使用PICRUSt2软件预测差异微生物功能。
CRC组和对照组在肠道微生物群的组成和丰度上均存在差异。厚壁菌门和拟杆菌门是两组中最丰富的菌门，而疣微菌门在CRC组中显著更丰富。对照组中[此处原文缺失具体菌名]、[此处原文缺失具体菌名]和[此处原文缺失具体菌名]更为丰富；两组间有18个属的丰度存在显著差异，这些属涉及21条代谢途径。有和无淋巴结转移的CRC患者肠道微生物群的分布和丰度存在显著差异；在属水平上，淋巴结转移患者中[此处原文缺失具体菌名]和[此处原文缺失具体菌名]的丰度显著更高，而[此处原文缺失具体菌名]、[此处原文缺失具体菌名]和[此处原文缺失具体菌名]的丰度显著更低。
与健康个体相比，CRC患者的肠道微生物群发生了改变，微生物群的特定变化与肿瘤分期、淋巴结受累及肿瘤分化等临床和病理特征相关。我们的研究结果在一定程度上阐述了肠道微生物群与CRC之间的联系。
