Department of Genenal Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, China.
Bioengineered. 2021 Dec;12(1):7046-7060. doi: 10.1080/21655979.2021.1972077.
The microbial diversity and communities in the excrement of healthy and patients suffered from cancer were identified by 16SrDNA sequencing performed on the Illumina Hi Seq sequencing platform. The microbial difference was also analyzed. The sequencing results showed high quality of the data, and the microbial communities were more various in the excrement of cancer patients. And the abundance of Firmicutes phylum was significantly reduced in cancer group. The phylum of Fermicutes, Bacteroidetes in cancer group are significantly down-regulated and up-regulated compared with normal group. The species of and are significantly lower in cancer group than that in normal group (< 0.05). The species of and are significantly higher in the cancer group than that in normal group. The comparative results indicated that beneficial bacterium significantly decreased in colorectal cancer (CRC) group, and harmful bacterium significantly increased in the colon cancer group, meanwhile the acidity, sugar increased whereas the oxygen content decreased to facilitate the growth of harmful bacterium. The results would provide microbial approaches for the treatment of colon cancer by the intake of beneficial microbial communities.
通过在 Illumina HiSeq 测序平台上进行 16SrDNA 测序，鉴定了健康人和癌症患者粪便中的微生物多样性和群落。还分析了微生物差异。测序结果显示数据质量很高，癌症患者粪便中的微生物群落更加多样化。并且癌症组中厚壁菌门的丰度显著降低。与正常组相比，癌症组中的拟杆菌门和厚壁菌门显著下调和上调。癌症组中 和 的丰度明显低于正常组(<0.05)。癌症组中 和 的丰度明显高于正常组。比较结果表明，结直肠癌（CRC）组中有益菌显著减少，而有害菌显著增加，同时酸度、糖分增加，而含氧量减少，有利于有害菌生长。该结果将为通过摄入有益微生物群落治疗结肠癌提供微生物方法。
