Yu Liyang, Tian Yujuan, Wang Jinxin, Wang Dongsheng, Wang Xuan, Zhang Haie, Zhang Jingzheng, Wang Xiangyu
Engineering Research Center of Chestnut Industry Technology, Ministry of Education, Hebei Normal University of Science and Technology, Qinhuangdao, 066004, China.
Shijiazhuang Institute of Pomology, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang, Hebei, 050061, China.
BMC Plant Biol. 2025 Jun 9;25(1):778. doi: 10.1186/s12870-025-06829-8.
The DnaJ gene family plays crucial roles in the plant abiotic stress response. Although the DnaJ gene family has been extensively characterized in various plants, its distribution in the Castanea mollissima genome is still unclear.
In this study, 78 CmDnaJ genes were identified and characterized in the C. mollissima genome, divided into three subfamilies based on the phylogeny analysis. The abundance of cis-acting elements related to temperature stress in the promoter regions of the CmDnaJ genes suggested a close relationship between this family and temperature stress response. Dispersed duplication was identified as the main driving force behind the expansion of the CmDnaJ family. RNA-seq data from 192 runs across nine projects were analyzed, revealing the potential roles of CmDnaJ genes in the growth, development, and environmental stress response of C. mollissima. Weighted gene co-expression network analysis showed that CmDnaJ27 and CmDnaJ34 were located in the red module and significantly correlated with temperature stimulus-response. RT-qPCR experiments and subcellular localization validated the expression levels and specific locations of CmDnaJ genes under cold and heat stresses. Overexpression of CmDnaJ27 in Nicotiana tabacum significantly reduces its tolerance to cold and heat stresses.
This study provides insights into the important roles of the DnaJ gene family in C. mollissima growth, development, and response to environmental stress, and provides reference to the research of CmDnaJs in cold and heat stresses response.
DnaJ基因家族在植物非生物胁迫响应中发挥着关键作用。尽管DnaJ基因家族已在多种植物中得到广泛表征，但其在板栗基因组中的分布仍不清楚。
在本研究中，在板栗基因组中鉴定并表征了78个CmDnaJ基因，根据系统发育分析将其分为三个亚家族。CmDnaJ基因启动子区域中与温度胁迫相关的顺式作用元件的丰度表明该家族与温度胁迫响应之间存在密切关系。分散重复被确定为CmDnaJ家族扩张的主要驱动力。分析了来自九个项目的192次运行的RNA-seq数据，揭示了CmDnaJ基因在板栗生长、发育和环境胁迫响应中的潜在作用。加权基因共表达网络分析表明，CmDnaJ27和CmDnaJ34位于红色模块中，与温度刺激响应显著相关。RT-qPCR实验和亚细胞定位验证了CmDnaJ基因在冷胁迫和热胁迫下的表达水平和特定位置。在烟草中过表达CmDnaJ27显著降低了其对冷胁迫和热胁迫的耐受性。
本研究深入了解了DnaJ基因家族在板栗生长、发育和对环境胁迫响应中的重要作用，并为CmDnaJs在冷胁迫和热胁迫响应中的研究提供了参考。
