Izadi F, Ramalakshmi S
Department of Pharmacy Practice, K.K. College of Pharmacy, The Tamil Nadu Dr. M.G.R. Medical University, Chennai, India.
Arch Razi Inst. 2024 Jun 30;79(3):593-600. doi: 10.32592/ARI.2024.79.3.593. eCollection 2024 Jun.
Cancer, a disease threatening human life, is caused by the disturbance of the normal cell cycle, which results in the spontaneous growth of normal and malignant cells, the lack of differentiation between the two, and consequently malignant growths. Nowadays, various synthetic agents are applied for cancer therapy; nevertheless, reports have confirmed that these chemical agents are associated with various adverse complications. This experimental study was designed to assess the anti-tumor activities of zinc nanoparticles (ZnNPs) green synthesized by the () extract against Ehrlich solid tumors (EST) in mice. To induce the EST model, 0.2 ml of cell suspension was intramuscularly injected into the right thigh of the mice. Five days post-injection, the mice were assigned to five groups (eight mice each): EST mice treated with normal saline, EST mice orally treated with ZnNPs 10 mg/kg/day, EST mice orally treated with ZnNPs 25 mg/kg/day, and EST mice orally treated with ZnNPs 50 mg/kg/day for 14 days. Afterward, the volume of the tumor, tumor growth inhibition, body weight, tumor markers, oxidant/antioxidant markers, and tumor necrosis factor-alpha level were assessed in the tested mice. The results showed that after the treatment of EST mice with cyclophosphamide and ZnNPs at 10, 25, and 50 mg/kg, the volume of the tumor, and the serum amount of tumor markers (alpha-fetoprotein and carcinoembryonic antigen) were significantly reduced (<0.001). It was found that ZnNPs at 10, 25, and 50 mg/kg markedly declined oxidative markers and increased the level of antioxidant enzymes (superoxide dismutase and glutathione peroxidase), compared to the control group, which received normal saline (<0.001). To conclude, this study reported the unveiling of the anti-tumor activity of ZnNPs green synthesized by the extract, mainly at a dose of 50 mg/kg against EST in mice. However, further supplementary studies are required to clarify all the anti-tumor aspects of these nanoparticles.
癌症，一种威胁人类生命的疾病，是由正常细胞周期紊乱引起的，这导致正常细胞和恶性细胞的自发生长、两者之间缺乏分化，进而导致恶性生长。如今，各种合成药物被用于癌症治疗；然而，报告证实这些化学药物会引发各种不良并发症。本实验研究旨在评估由（）提取物绿色合成的锌纳米颗粒（ZnNPs）对小鼠艾氏实体瘤（EST）的抗肿瘤活性。为诱导EST模型，将0.2 ml细胞悬液肌肉注射到小鼠右大腿。注射后五天，将小鼠分为五组（每组八只）：用生理盐水治疗的EST小鼠、口服10 mg/kg/天ZnNPs治疗的EST小鼠、口服25 mg/kg/天ZnNPs治疗的EST小鼠、口服50 mg/kg/天ZnNPs治疗的EST小鼠，持续14天。之后，对受试小鼠的肿瘤体积、肿瘤生长抑制、体重、肿瘤标志物、氧化/抗氧化标志物以及肿瘤坏死因子-α水平进行评估。结果显示，用环磷酰胺和10、25、50 mg/kg的ZnNPs治疗EST小鼠后，肿瘤体积以及肿瘤标志物（甲胎蛋白和癌胚抗原）的血清含量显著降低（<0.001）。发现与接受生理盐水的对照组相比，10、25、50 mg/kg的ZnNPs显著降低了氧化标志物水平，并提高了抗氧化酶（超氧化物歧化酶和谷胱甘肽过氧化物酶）的水平（<0.001）。总之，本研究报告了由提取物绿色合成的ZnNPs的抗肿瘤活性，主要是在50 mg/kg剂量下对小鼠EST的活性。然而，需要进一步的补充研究来阐明这些纳米颗粒所有的抗肿瘤方面。
