Yuan Ke-Yu, Gu Yao-Hua, Pei Yi-Hao, Yu Si-Yu, Li Tian-Zhou, Feng Tian, Liu Yu, Tian Jianbo, Miao Xiaoping, Xiong Jun, Hu Min, Yuan Bi-Feng
Department of Occupational and Environmental Health, School of Public Health, Wuhan University, Department of Radiation and Medical Oncology, Zhongnan Hospital of Wuhan University, Wuhan 430071, China; Research Center of Public Health, Renmin Hospital of Wuhan University, Wuhan University, Wuhan 430060, China.
Department of Occupational and Environmental Health, School of Public Health, Wuhan University, Department of Radiation and Medical Oncology, Zhongnan Hospital of Wuhan University, Wuhan 430071, China; School of Nursing, Wuhan University, Wuhan 430071, China.
J Hazard Mater. 2025 Mar 15;486:136970. doi: 10.1016/j.jhazmat.2024.136970. Epub 2024 Dec 24.
Prenatal exposure to hazardous environmental pollutants is a critical global concern due to their confirmed presence in umbilical cord blood, indicating the ability of pollutants to cross the placental barrier and expose the fetus to harmful compounds. However, the transplacental transfer efficiencies (TTEs) of many pollutants remain underexplored. Herein, we developed a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to quantitatively analyze 91 environmental pollutants, including 13 bisphenols (BPs), 18 organophosphorus flame retardants (OPFRs), 7 brominated and other flame retardants (BFRs), 34 phthalates (PAEs), and 19 per- and polyfluoroalkyl substances (PFASs), in paired maternal and cord serums. 38 pollutants were detected in serums, including 5 BPs, 13 OPFRs, 2 BFRs, 4 PAEs, and 14 PFASs. Among the detected pollutants, bisphenol A (BPA) exists in the highest concentration (GM: 10.92 ng/mL in maternal serums and 12.66 ng/mL in cord serums), followed by tris(1,3-dichloro-2-propyl) phosphate (TDCIPP), perfluorooctanoic acid (PFOA), and 4,4'-(1,3-phenylenediisopropylidene) bisphenol (BPM). The exposure concentrations of the same type of pollutants were highly correlated between maternal and cord serums. Perfluorohexanoic acid (PFHxA) had the highest TTE value (5.526), while perfluorooctane sulfonic acid (PFOS) had the lowest (0.206). TTEs of PFOS and perfluorononanoic acid (PFNA) were higher for female newborns, whereas TTEs of perfluorohexadecanoic acid (PFHxDA) and perfluorodecane sulfonic acid (PFDS) were higher for male newborns. Moreover, the expression levels of the transplacental transporters ABCA1, ABCC2, ABCC3, ABCC4, ABCG1, SLCO3A1, and SLC22A3 were associated with the transplacental transfer of triphenyl phosphate (TPHP), TDCIPP, di-n-propyl phthalate (DPRP), perfluoroundecanoic acid (PFUnDA), perfluorotridecanoic acid (PFTrDA), and PFOS. Further research is essential to unveil the mechanisms involved in the transplacental transfer of environmental pollutants, ultimately boosting our comprehension of their impact on fetal health and birth outcomes.
由于在脐带血中已证实存在有害环境污染物，产前暴露于这些污染物是一个至关重要的全球问题，这表明污染物能够穿过胎盘屏障，使胎儿接触到有害化合物。然而，许多污染物的经胎盘转移效率（TTEs）仍未得到充分研究。在此，我们开发了一种液相色谱 - 串联质谱（LC-MS/MS）方法，用于定量分析配对的母体血清和脐带血清中的91种环境污染物，包括13种双酚（BPs）、18种有机磷阻燃剂（OPFRs）、7种溴化及其他阻燃剂（BFRs）、34种邻苯二甲酸酯（PAEs）和19种全氟和多氟烷基物质（PFASs）。在血清中检测到38种污染物，包括5种BPs、13种OPFRs、2种BFRs、4种PAEs和14种PFASs。在检测到的污染物中，双酚A（BPA）的浓度最高（母体血清中几何均值：10.92 ng/mL，脐带血清中为12.66 ng/mL），其次是磷酸三（1,3 - 二氯 - 2 - 丙基）酯（TDCIPP）、全氟辛酸（PFOA）和4,4'-（1,3 - 亚苯基二异丙叉）双酚（BPM）。母体血清和脐带血清中同一类型污染物的暴露浓度高度相关。全氟己酸（PFHxA）的TTE值最高（5.526），而全氟辛烷磺酸（PFOS）最低（0.206）。PFOS和全氟壬酸（PFNA）在女新生儿中的TTE较高，而全氟十六烷酸（PFHxDA）和全氟癸烷磺酸（PFDS）在男新生儿中的TTE较高。此外，经胎盘转运蛋白ABCA1、ABCC2、ABCC3、ABCC4、ABCG1、SLCO3A1和SLC22A3的表达水平与磷酸三苯酯（TPHP）、TDCIPP、邻苯二甲酸二正丙酯（DPRP）、全氟十一烷酸（PFUnDA）、全氟十三烷酸（PFTrDA）和PFOS的经胎盘转移有关。进一步的研究对于揭示环境污染物经胎盘转移所涉及的机制至关重要，最终有助于我们更好地理解它们对胎儿健康和出生结局的影响。
