Suzuki-Nagata Shino, Mase Nobuyuki, Kozuka Tomoki, Ng Jack C, Suzuki Tetsuya
Department of Applied Chemistry and Biochemical Engineering, Faculty of Engineering, Shizuoka University, Hamamatsu, Shizuoka, Japan.
Queensland Alliance for Environmental Health Sciences (QAEHS), The University of Queensland, Brisbane, Australia.
Biosci Biotechnol Biochem. 2025 Mar 24;89(4):638-648. doi: 10.1093/bbb/zbae210.
Microalgae have been explored as a viable alternative food source. Among them, Euglena gracilis stands out as a promising single-cell algae. However, the challenge lies in developing more efficient and cost-effective methods for industrial mass production of E. gracilis under controlled culture conditions. Our research aimed to address this by investigating the role of nanotechnology in using fine to ultra-fine bubble CO2 (FB-CO2)-ranging from micrometer to nanometer size-as feeding material to promote cell harvest of E. gracilis Z in autotrophic culture conditions. Our findings suggest that feeding E. gracilis Z with FB-CO2 increased cell growth and chlorophyll content in autotrophic culture conditions. The promotion effect can be attributed to the provision of non-ionized carbon dioxide to the photosynthetic system, which was further enhanced by the dispersion of FB-CO2 in the culture media under acidic conditions.
微藻已被探索作为一种可行的替代食物来源。其中，纤细裸藻作为一种有前景的单细胞藻类脱颖而出。然而，挑战在于开发更高效且具成本效益的方法，以便在可控培养条件下对纤细裸藻进行工业大规模生产。我们的研究旨在通过研究纳米技术在使用从微米到纳米尺寸的细至超细气泡二氧化碳（FB-CO2）作为进料来促进自养培养条件下纤细裸藻Z的细胞收获方面的作用来解决这一问题。我们的研究结果表明，在自养培养条件下用FB-CO2喂养纤细裸藻Z可增加细胞生长和叶绿素含量。这种促进作用可归因于向光合系统提供了非离子化二氧化碳，在酸性条件下FB-CO2在培养基中的分散进一步增强了这种作用。
