Lin Tainan, Hu Jianzhang, Wen Qian, Liu Xiaoting, Lin Jinghua, Shi Qiaomei, Lin Miao, Huang Weifu
Department of Ophthalmology, Fujian Medical University Union Hospital, Fuzhou, China.
Department of Ophthalmology, Fujian Provincial Governmental Hospital, Fuzhou, China.
Front Med (Lausanne). 2024 Dec 17;11:1502612. doi: 10.3389/fmed.2024.1502612. eCollection 2024.
This study aims to investigate the protective effects of docosahexaenoic acid (DHA) combined with bilberry extract (BE) on myopic guinea pigs. In total, 105 healthy pigmented guinea pigs aged 2 weeks were selected and randomly divided into five groups. The normal control (NC) group received no treatment, while the experimental groups wore -6.0D lenses on the right eye to establish an animal model of lens-induced myopia (LIM). These groups were further divided based on different treatments: normal feeding, DHA treatment, BE treatment, and combined DHA + BE treatment. Refractive error and axial length for both eyes were measured before modeling, after 4 weeks of modeling, and after 8 weeks of treatment. Fundus examination was performed, and choroidal thickness, choroidal vascularity index (CVI), maximal mixed response in dark adaptation (Max-ERG), and cone cell response in light adaptation (Cone-ERG) were measured. After 8 weeks of treatment, we observed a significant reduction in refractive error and shortening of axial length, improvement in fundus condition, and increased choroidal thickness and CVI in the LIM + DHA + BE group. Electroretinogram (ERG) showed that the amplitudes of a-wave and b-wave were enhanced in both Max-ERG and Cone-ERG tests. The LIM + DHA + BE group exhibited superior effects compared to the LIM + DHA group and the LIM + BE group. The combination of DHA and BE delayed the progression of LIM in guinea pigs and was more effective than DHA or BE alone. The synergistic effect of DHA and BE offers a new approach to the prevention and treatment of myopia.
本研究旨在探讨二十二碳六烯酸（DHA）联合越橘提取物（BE）对近视豚鼠的保护作用。总共选取了105只2周龄的健康有色豚鼠，并随机分为五组。正常对照组（NC）不接受任何处理，而实验组右眼佩戴-6.0D镜片以建立透镜诱导性近视（LIM）动物模型。这些组根据不同处理进一步细分：正常喂养、DHA处理、BE处理以及DHA+BE联合处理。在建模前、建模4周后以及处理8周后测量双眼的屈光不正和眼轴长度。进行眼底检查，并测量脉络膜厚度、脉络膜血管指数（CVI）、暗适应最大混合反应（Max-ERG）以及明适应锥体细胞反应（Cone-ERG）。处理8周后，我们观察到LIM+DHA+BE组的屈光不正显著降低，眼轴长度缩短，眼底状况改善，脉络膜厚度和CVI增加。视网膜电图（ERG）显示，在Max-ERG和Cone-ERG测试中，a波和b波的振幅均增强。与LIM+DHA组和LIM+BE组相比，LIM+DHA+BE组表现出更优的效果。DHA和BE的联合延缓了豚鼠LIM的进展，且比单独使用DHA或BE更有效。DHA和BE的协同作用为近视的预防和治疗提供了一种新方法。
