Taniguchi Kentaro, Okumura Naoya, Jinno Naoya, Seiyama Akitoshi, Shimouchi Akito
Department of Medical Laboratory Science, Faculty of Health Sciences, Kochi Gakuen University, Kochi, Kochi, Japan.
Department of Bioscience, Nagahama Institute of Bio-Science and Technology, Nagahama, Shiga, Japan.
MethodsX. 2024 Dec 5;14:103085. doi: 10.1016/j.mex.2024.103085. eCollection 2025 Jun.
This study aimed to assess fatigue using a noninvasive, quantitative, and simple method using salivary chromogranin A (CgA). In total, 171 adults participated in this study. We used the Cornell Medical Index (CMI) as a questionnaire that included a fatigability section. The high-fatigability group had significantly ( < 0.01, = 0.21) lower levels of salivary CgA after waking up than those of the low-fatigability group. Before sleep, participants with high stress on the musculoskeletal system (CMI E, = 0.008, = 0.18), skin (CMI F, = 0.017, = 0.16), nervous system (CMI G, = 0.019, = 0.16), habit (CMI L, = 0.028, = 0.16), sensitivity (CMI P, = 0.022, = 0.16), and anger (CMI Q, = 0.011, = 0.16) had significantly lower CgA levels than those of low stress. In conclusion, we found that salivary CgA levels after waking up could reflect fatigability, and those before night sleep could reflect chronic physical complaints.•Assessment of Salivary CgA levels using an enzyme-linked immunosorbent assay.•Routine saliva analysis could enable allow detection of chronic fatigue or stress, leading to timely lifestyle adjustments or interventions to maintain overall well-being.
本研究旨在使用唾液嗜铬粒蛋白A（CgA），通过一种非侵入性、定量且简单的方法来评估疲劳。共有171名成年人参与了本研究。我们使用康奈尔医学指数（CMI）作为包含疲劳易感性部分的问卷。高疲劳易感性组醒来后的唾液CgA水平显著低于低疲劳易感性组（<0.01， = 0.21）。在睡眠前，肌肉骨骼系统（CMI E， = 0.008， = 0.18）、皮肤（CMI F， = 0.017， = 0.16）、神经系统（CMI G， = 0.019， = 0.16）、习惯（CMI L， = 0.028， = 0.16）、敏感性（CMI P， = 0.022， = 0.16）和愤怒（CMI Q， = 0.011， = 0.16）方面压力较高的参与者的CgA水平显著低于压力较低的参与者。总之，我们发现醒来后的唾液CgA水平可以反映疲劳易感性，而夜间睡眠前的唾液CgA水平可以反映慢性身体不适。
•使用酶联免疫吸附测定法评估唾液CgA水平。
•常规唾液分析能够检测慢性疲劳或压力，从而及时调整生活方式或进行干预以维持整体健康。
