as a prognostic biomarker and potential therapeutic target in kidney renal clear cell carcinoma.

BACKGROUND

Progestin And AdipoQ Receptor Family Member VI () plays a significant role in the non-genomic effects of rapid steroid responses and is abnormally expressed in various tumors. However, its biological function in kidney renal clear cell carcinoma (KIRC) and its potential as a therapeutic target remain underexplored.

METHODS

In this study, was identified as a critical oncogene by WGCNA algorithm and differential gene expression analysis using TCGA - KIRC and GSE15641 data. The differences in expression and its association with KIRC survival outcomes were investigated, and transcriptomic data were used to further elucidate 's biological functions. Moreover, XCELL and single - cell analysis assessed the correlation between expression and immune infiltration. TIDE algorithm was used to assess how well various patient cohorts responded to immune checkpoint therapy. Finally, the role of in the development of KIRC was verified through EdU, scratch assays, and Transwell assays.

RESULTS

Our findings suggest that elevated expression of is linked to a poor prognosis for KIRC patients. Functional enrichment analysis demonstrated that is primarily involved in angiogenesis and pluripotent stem cell differentiation, which are crucial in mediating the development of KIRC. Additionally, we established a ceRNA network that is directly related to overall prognosis, further supporting the role of as a prognostic biomarker for KIRC.

CONCLUSION

Using both computational and experimental methods, this study leads the charge in discovering and verifying as a prognostic biomarker and possible therapeutic target for KIRC. In the future, to determine its molecular mechanism in KIRC carcinogenesis, more research will be carried out.