Shakeri Abolfazl, Mashhadi Esmaeilabadi Aliakbar, Soheili Vahid, Mottaghipisheh Javad, Emami Seyed Ahmad, Akhtari Javad, Tayarani-Najaran Zahra
Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Pharmaceutical Control, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
Curr Med Mycol. 2024 May 7;10. doi: 10.22034/cmm.2024.345183.1499. eCollection 2024.
species are well-known antifungal medicinal plants. (Apiaceae family) is a rarely investigated plant endemic to Iran. The present study aimed to assess the antifungal, antibacterial, antioxidant, and cytotoxic activities of root extracts of different plants.
The methanolic crude extract (MeOH) and its sub-fractions, including petroleum ether (PE), dichloromethane (DCM), ethyl acetate (EtOAc), and n-butanol (n-BuOH) were prepared.
Results from the antifungal and antibacterial activities of fractions indicated remarkable antifungal effects against with minimum inhibitory concentration and minimum bactericidal concentration values of 10 µg/mL; however, no cytotoxicity was observed in the case of selected cancer cells. Moreover, methanolic soluble fractions showed good antiradical effects evaluated DPPH and -carotene bleaching tests possessing half-maximal inhibitory concentration (IC) of 20.11 and 41.32 µg/mL, respectively, though it was less effective than positive controls ascorbic acid (8.47 and 31.71 µg/mL, respectively) and butylated hydroxytoluene (IC: 10.29 and 33.55 µg/mL, respectively).
It can be concluded that strong antifungal and antioxidant activities without notable cytotoxicity, suggest the potential safety of the plant to be used as a natural antifungal remedy as well as a preservative in the food industry.
某些物种是著名的抗真菌药用植物。（伞形科）是一种原产于伊朗但鲜少被研究的植物。本研究旨在评估不同植物根提取物的抗真菌、抗菌、抗氧化和细胞毒性活性。
制备了甲醇粗提物（MeOH）及其亚组分，包括石油醚（PE）、二氯甲烷（DCM）、乙酸乙酯（EtOAc）和正丁醇（n - BuOH）。
各组分的抗真菌和抗菌活性结果表明，对[具体菌种未提及]具有显著的抗真菌作用，最小抑菌浓度和最小杀菌浓度值为10μg/mL；然而，在所选癌细胞中未观察到细胞毒性。此外，甲醇可溶组分在DPPH和β - 胡萝卜素漂白试验中显示出良好的抗自由基作用，半最大抑制浓度（IC）分别为20.11和41.32μg/mL，不过其效果不如阳性对照抗坏血酸（分别为8.47和31.71μg/mL）和丁基羟基甲苯（IC：分别为10.29和33.55μg/mL）。
可以得出结论，该植物具有强大的抗真菌和抗氧化活性且无明显细胞毒性，表明其作为天然抗真菌药物以及食品工业中的防腐剂具有潜在的安全性。
