Liyanage Senal, Guo Jia
Biodesign Institute and School of Molecular Sciences, Arizona State University, Tempe, Arizona, USA.
Histol Histopathol. 2025 Aug;40(8):1173-1184. doi: 10.14670/HH-18-861. Epub 2024 Dec 13.
Recent advancements in single-cell spatial proteomics have revolutionized our ability to elucidate cellular signaling networks and their implications in health and disease. This review examines these cutting-edge technologies, focusing on mass spectrometry (MS) imaging and multiplexed immunofluorescence (mIF). Such approaches allow high-resolution protein profiling at the single-cell level, revealing intricate cellular heterogeneity, spatial organization, and protein functions within their native cellular contexts. MS imaging techniques offer unprecedented high-dimensional resolution and provide detailed insights into their subcellular protein localization and abundance. mIF enables rapid and high-throughput protein profiling, enhancing its accessibility for diverse research and clinical applications. This review assesses the current challenges associated with these methodologies and also discusses the potential solutions to overcome these obstacles. The integration of spatial proteomics with other systems biology approaches holds great promise for enhancing our understanding of complex biological systems. It could also lead to significant advancements in molecular diagnostics and personalized treatment strategies.
单细胞空间蛋白质组学的最新进展彻底改变了我们阐明细胞信号网络及其在健康和疾病中的意义的能力。本综述探讨了这些前沿技术，重点关注质谱（MS）成像和多重免疫荧光（mIF）。此类方法允许在单细胞水平进行高分辨率蛋白质分析，揭示其天然细胞环境中复杂的细胞异质性、空间组织和蛋白质功能。MS成像技术提供了前所未有的高维分辨率，并对亚细胞蛋白质定位和丰度提供了详细的见解。mIF能够实现快速且高通量的蛋白质分析，提高了其在各种研究和临床应用中的可及性。本综述评估了与这些方法相关的当前挑战，并讨论了克服这些障碍的潜在解决方案。空间蛋白质组学与其他系统生物学方法的整合有望加深我们对复杂生物系统的理解。它还可能在分子诊断和个性化治疗策略方面带来重大进展。
