Zhang Hongli, Xu Huan, Luo Haihua, Li Xiangming, Gao Tian, Wu Qiong, Zeng Danlin
Key Laboratory of Coal Conversion and New Carbon Materials of Hubei Province & Institute of Advanced Materials and Nanotechnology, School of Chemistry and Chemical Engineering, School of Medicine, Wuhan University of Science and Technology, Wuhan, China.
Key Laboratory of Coal Conversion and New Carbon Materials of Hubei Province & Institute of Advanced Materials and Nanotechnology, School of Chemistry and Chemical Engineering, School of Medicine, Wuhan University of Science and Technology, Wuhan, China.
Int J Biol Macromol. 2025 Mar;293:139428. doi: 10.1016/j.ijbiomac.2024.139428. Epub 2024 Dec 31.
Bacterial infection of skin wounds leads to serious health problems, including skin defects, inflammatory pain, and even death. To meet the requirements for successful treatment of complicated wounds, a multifunctional dressing is thus highly desirable. In this work, a thermosensitive hydrogel dressing (HBCA) exhibiting injectability, adaptiveness and mild photothermal antibacterial activity was developed for effective infected wound treatment. The HBCA hydrogel was constructed by integrating Ag NPs-incorporated quaternized chitin (DQCA) into the matrix of hydroxybutyl chitin. Laser irradiation could activate the photothermal DQCA to generate heat and rapidly induce the destruction of the bacterial integrity. Meanwhile, the release of DQCA can be accelerated by laser-mediated heating in a controllable manner, quickly reaching the effective concentration and leading to bacterial inactivation in a combined way. The HBCA hydrogel possessed broad-spectrum antibacterial and antibiofilm effect under mild temperature (~48 °C), through combining photothermal therapy (PTT) with chemotherapeutic DQCA. Furthermore, the mouse full-thickness MRSA-infected wound healing test demonstrated that the HBCA hydrogel could accelerate wound regeneration by reducing the inflammatory response, promoting re-epithelialization as well as collagen maturation. This work presents an efficacious and straightforward antibacterial hydrogel dressing that incorporates mild-temperature PTT, particularly in the realm of multidrug-resistant bacterial infection therapies.
皮肤伤口的细菌感染会导致严重的健康问题，包括皮肤缺损、炎症性疼痛，甚至死亡。因此，为了满足成功治疗复杂伤口的要求，一种多功能敷料是非常必要的。在这项工作中，开发了一种具有可注射性、适应性和温和光热抗菌活性的热敏水凝胶敷料（HBCA），用于有效治疗感染伤口。HBCA水凝胶是通过将掺入银纳米颗粒的季铵化壳聚糖（DQCA）整合到羟丁基壳聚糖基质中构建而成的。激光照射可以激活光热DQCA产生热量，并迅速诱导细菌完整性的破坏。同时，激光介导的加热可以以可控的方式加速DQCA的释放，迅速达到有效浓度，并以联合方式导致细菌失活。通过将光热疗法（PTT）与化疗药物DQCA相结合，HBCA水凝胶在温和温度（约48°C）下具有广谱抗菌和抗生物膜作用。此外，小鼠全层耐甲氧西林金黄色葡萄球菌感染伤口愈合试验表明，HBCA水凝胶可以通过减轻炎症反应、促进上皮再形成以及胶原蛋白成熟来加速伤口再生。这项工作提出了一种有效且直接的抗菌水凝胶敷料，其结合了温和温度下的PTT，特别是在多重耐药细菌感染治疗领域。
