Hebei Key Laboratory of Functional Polymers, School of Chemical Engineering and Technology, Hebei University of Technology, 5340 Xiping Road, Beichen District, Tianjin, 300130, P. R. China.
Tianjin Key Laboratory of Biomaterial Research, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences and Peking Union Medical College, 236 Baidi Road, Nankai District, Tianjin, 300192, P. R. China.
Adv Sci (Weinh). 2023 Apr;10(11):e2206585. doi: 10.1002/advs.202206585. Epub 2023 Feb 12.
Bacterial infection often induces chronic repair of wound healing owing to aggravated inflammation. Hydrogel dressing exhibiting intrinsic antibacterial activity may substantially reduce the use of antibiotics for infected wound management. Hence, a versatile hydrogel dressing (rGB/QCS/PDA-PAM) exhibiting skin adaptiveness on dynamic wounds and mild photothermal antibacterial activity is developed for safe and efficient infected wound treatment. Phenylboronic acid-functionalized graphene (rGB) and oxadiazole-decorated quaternary carboxymethyl chitosan (QCS) are incorporated into a polydopamine-polyacrylamide (PDA-PAM) network with multiple covalent and noncovalent bonds, which conferred the hydrogel with flexible mechanical properties, strong tissue adhesion and excellent self-healing ability on the dynamic wounds. Moreover, the glycocalyx-mimicking phenylboronic acid on the surface of rGB enables the hydrogel to specifically capture bacteria. The enhanced membrane permeability of QCS enhanced bacterial vulnerability to photothermal therapy（PTT）, which is demonstrated by efficient mild PTT antibacteria against methicillin-resistant Staphylococcus aureus in vitro and in vivo at temperatures of <49.6 °C. Consequently, the hydrogel demonstrate accelerated tissue regeneration on MRSA-infected wound in vivo, with an intact epidermis, abundant collagen deposition and prominent angiogenesis. Therefore, rGB/QCS/PDA-PAM is a versatile hydrogel dressing exhibiting inherent antibacterial activity and has considerable potential in treating wounds infected with drug-resistant bacteria.
细菌感染常因炎症加重而导致慢性伤口愈合修复。具有固有抗菌活性的水凝胶敷料可显著减少感染性伤口管理中抗生素的使用。因此，开发了一种多功能水凝胶敷料（rGB/QCS/PDA-PAM），它在动态伤口上具有皮肤适应性和温和的光热抗菌活性，可安全有效地治疗感染性伤口。苯硼酸功能化石墨烯（rGB）和恶二唑修饰的季铵化壳聚糖（QCS）被掺入具有多个共价和非共价键的聚多巴胺-聚丙烯酰胺（PDA-PAM）网络中，赋予水凝胶在动态伤口上具有灵活的机械性能、强大的组织粘附性和出色的自修复能力。此外，rGB 表面的糖萼模拟苯硼酸使水凝胶能够特异性地捕获细菌。QCS 的增强的膜通透性增强了细菌对光热疗法（PTT）的敏感性，这在体外和体内实验中得到了证明，在<49.6°C 的温度下，对耐甲氧西林金黄色葡萄球菌（MRSA）具有高效的温和 PTT 抗菌作用。因此，水凝胶在 MRSA 感染的伤口中表现出加速的组织再生，具有完整的表皮、丰富的胶原蛋白沉积和明显的血管生成。因此，rGB/QCS/PDA-PAM 是一种具有固有抗菌活性的多功能水凝胶敷料，在治疗耐药菌感染的伤口方面具有很大的潜力。
