Huang Huang, Li Minyao, Song Shuo, Feng Shiyu, Feng Xiaoyang, Liu Yu, Yang Pingchang, Zheng Pengyuan
Department of Gastroenterology, Fifth Affiliated Hospital, Zhengzhou University, Zhengzhou, China.
Department of General Practice Medicine, Third Affiliated Hospital of Shenzhen University, Shenzhen, China.
Sci Rep. 2025 Jan 2;15(1):196. doi: 10.1038/s41598-024-84079-8.
The deregulation of immune responses is what causes food allergy (FA) to occur. FA's cause is still unknown. The goal of this study is to investigate the mechanism how the impaired production of IL-10 occurs in peripheral naive B cells of patients with FA. Samples from patients with FA and healthy controls (HC) were used to isolate CD19 CD45R naive B cells from peripheral blood mononuclear cells (PBMC). Lipopolysaccharide (LPS) exposure was used to assess the expression of interleukin-10 (IL-10) in B cells. Although the FA and HC groups had similar total B cell counts, the FA patients had fewer IL-10 B cell counts than the HC group. In peripheral B cells, the concentrations of IL-10 were inversely related to the concentrations of specific IgE, Th2 and Th1 cytokines in the serum. In patients with FA, peripheral B cells experienced impaired immune-suppressive functions. Galectin-9 could restore the defective induction of IL-10 expression in naive B cells of FA patients. In conclusion, FA patients with naive B cells experience impaired IL-10 induction. The induction of IL-10 in naive B cells of FA patients can be restored by galectin-9 treatment, which triggers B cells to differentiate into immune regulatory B cells.
免疫反应失调是导致食物过敏（FA）发生的原因。FA的病因尚不清楚。本研究的目的是探讨FA患者外周幼稚B细胞中白细胞介素-10（IL-10）产生受损的机制。采用FA患者和健康对照（HC）的样本从外周血单核细胞（PBMC）中分离CD19 CD45R幼稚B细胞。通过脂多糖（LPS）刺激来评估B细胞中白细胞介素-10（IL-10）的表达。虽然FA组和HC组的总B细胞计数相似，但FA患者的IL-10阳性B细胞计数低于HC组。在外周B细胞中，IL-10的浓度与血清中特异性IgE、Th2和Th1细胞因子的浓度呈负相关。在FA患者中，外周B细胞的免疫抑制功能受损。半乳糖凝集素-9可以恢复FA患者幼稚B细胞中IL-10表达的缺陷诱导。总之，FA患者的幼稚B细胞中IL-10诱导受损。半乳糖凝集素-9治疗可恢复FA患者幼稚B细胞中IL-10的诱导，促使B细胞分化为免疫调节性B细胞。
