Department of Pediatric Allergy and Immunology, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China.
Department of Pharmacy, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China.
Phytother Res. 2021 Jun;35(6):3214-3225. doi: 10.1002/ptr.7034. Epub 2021 Feb 17.
Piper nigrum is extensively utilized because of its antioxidation, antiallergic, antitumor, antiinflammatory, antidiarrhea, and gastrointestinal protection. We attempted to indicate whether the Piper nigrum extract (PNE) could alleviate ovalbumin (OVA)-induced food allergy, and to explore its potential mechanism. An OVA-induced food allergy mouse model was established, and different concentrations of PNE were administrated. Symptoms of food allergy, levels of immunoglobulin E (IgE), mucosal mast cell protease-1 (mMCP-1), and intestine pathological changes were assessed. Additionally, the expressions of T helper (Th) 2, Th17 and regulatory T (Treg)-associated cytokines and the proportion of Th17 and Treg cells in CD4 T cells were measured. We found PNE attenuated symptoms of food allergy and decreased the levels of IgE and mMCP-1. In PNE group, the infiltration degree of inflammatory cells was ameliorated and the villi of small intestine were more complete. Moreover, the expressions of Th2 and Th17 cell-associated cytokines were down-regulated by PNE pretreatment, while the levels of Treg cell-associated cytokines were up-regulated. PNE decreased the number of Th17 cells, while increased the Tregs cells. PNE treatment dose-dependently improved the Th17/Treg balance. PNE plays a protective role in OVA-induced food allergy through inhibiting Th2 cell response and regulating the Th17/Treg balance.
胡椒因具有抗氧化、抗过敏、抗肿瘤、抗炎、抗腹泻和胃肠道保护等作用而被广泛应用。本研究旨在探讨胡椒提取物（PNE）是否可以缓解卵清蛋白（OVA）诱导的食物过敏，并探讨其潜在机制。建立 OVA 诱导的食物过敏小鼠模型，并给予不同浓度的 PNE。评估食物过敏症状、免疫球蛋白 E（IgE）、黏膜肥大细胞蛋白酶-1（mMCP-1）水平以及肠道病理变化。此外，还测量了 T 辅助（Th）2、Th17 和调节性 T（Treg）相关细胞因子的表达以及 CD4 T 细胞中 Th17 和 Treg 细胞的比例。结果发现，PNE 可减轻食物过敏症状，降低 IgE 和 mMCP-1 水平。PNE 组炎症细胞浸润程度减轻，小肠绒毛更完整。此外，PNE 预处理可下调 Th2 和 Th17 细胞相关细胞因子的表达，上调 Treg 细胞相关细胞因子的表达。PNE 可减少 Th17 细胞的数量，增加 Tregs 细胞的数量。PNE 治疗可改善 Th17/Treg 平衡，呈剂量依赖性。PNE 通过抑制 Th2 细胞反应和调节 Th17/Treg 平衡对 OVA 诱导的食物过敏发挥保护作用。
