Ghobadi Faezeh, Saadatmand Maryam, Simorgh Sara, Brouki Milan Peiman
Department of Chemical and Petroleum Engineering, Sharif University of Technology, Tehran, Iran.
Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.
Sci Rep. 2025 Jan 2;15(1):569. doi: 10.1038/s41598-024-84346-8.
We engineered a microfluidic platform to study the effects of bioactive glass nanoparticles (BGNs) on cell viability under static culture. We incorporated different concentrations of BGNs (1%, 2%, and 3% w/v) in collagen hydrogel (with a concentration of 3.0 mg/mL). The microfluidic chip's dimensions were optimized through fluid flow and mass transfer simulations. Collagen type I extracted from rat tail tendons was used as the main material, and BGNs synthesized by the sol-gel method were used to enhance the mechanical properties of the hydrogel. The extracted collagen was characterized using FTIR and SDS-PAGE, and BGNs were analyzed using XRD, FTIR, DLS, and FE-SEM/EDX. The structure of the collagen-BGNs hydrogels was examined using SEM, and their mechanical properties were determined using rheological analysis. The cytotoxicity of BGNs was assessed using the MTT assay, and the viability of fibroblast (L929) cells encapsulated in the collagen-BGNs hydrogel inside the microfluidic device was assessed using a live/dead assay. Based on all these test results, the L929 cells showed high cell viability in vitro and promising microenvironment mimicry in a microfluidic device. Collagen3-BGNs3 (Collagen 3 mg/mL + BGNs 3% (w/v)) was chosen as the most suitable sample for further research on a microfluidic platform.
我们设计了一个微流控平台，以研究生物活性玻璃纳米颗粒（BGNs）在静态培养下对细胞活力的影响。我们在胶原蛋白水凝胶（浓度为3.0 mg/mL）中加入了不同浓度的BGNs（1%、2%和3% w/v）。通过流体流动和传质模拟对微流控芯片的尺寸进行了优化。从大鼠尾腱中提取的I型胶原蛋白用作主要材料，通过溶胶-凝胶法合成的BGNs用于增强水凝胶的机械性能。使用傅里叶变换红外光谱（FTIR）和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）对提取的胶原蛋白进行表征，使用X射线衍射（XRD）、傅里叶变换红外光谱（FTIR）、动态光散射（DLS）和场发射扫描电子显微镜/能谱仪（FE-SEM/EDX）对BGNs进行分析。使用扫描电子显微镜（SEM）检查胶原蛋白-BGNs水凝胶的结构，并使用流变学分析确定其机械性能。使用MTT法评估BGNs的细胞毒性，并使用活/死检测法评估微流控装置内封装在胶原蛋白-BGNs水凝胶中的成纤维细胞（L929）的活力。基于所有这些测试结果，L929细胞在体外显示出高细胞活力，并在微流控装置中具有良好的微环境模拟能力。胶原蛋白3-BGNs3（胶原蛋白3 mg/mL + BGNs 3%（w/v））被选为最适合在微流控平台上进行进一步研究的样品。
