Huang Lidong, Gong Weiyu, Huang Guibin, Li Jingyi, Wu Jilin, Dong Yanmei
Department of Cariology and Endodontology, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral, Biomaterials and Digital Medical Devices, Beijing 100081, China.
Regen Biomater. 2023 Mar 24;10:rbad024. doi: 10.1093/rb/rbad024. eCollection 2023.
Bioactive glasses (BG) have been generally used in bone defects repair for its good osteoinductivity and osteoconductivity. However, the early angiogenesis of BG in the repair of large-sized bone defects may not be sufficient enough to support new bone formation, resulting in the failure of bone repair. Photobiomodulation (PBM) therapy, which is superior on promoting early angiogenesis, may contribute to the angiogenesis of BG and further enhance the repair of bone defects. Therefore, we applied BG and PBM in combination and preliminarily investigated their additive effects on bone regeneration both and . The results revealed that BG combined with PBM remarkably enhanced human bone marrow mesenchymal stem cells proliferation, osteogenic-related genes expression and mineralization, which was better than applying BG or PBM respectively. For studies, the histological staining results showed that BG induced new bone formation in the interior of defects and promoted new bone reconstruction at 6 weeks post-operation. The micro-computed tomography results further confirmed that BG combined with PBM accelerated bone formation and maturation, improved the speed and quality of bone regeneration, and promoted bone repair. In conclusion, with the optimum BG and PBM parameters, BG combined with PBM generated additive effects on promoting bone regeneration.
生物活性玻璃（BG）因其良好的骨诱导性和骨传导性，已普遍用于骨缺损修复。然而，BG在修复大型骨缺损时的早期血管生成可能不足以支持新骨形成，导致骨修复失败。光生物调节（PBM）疗法在促进早期血管生成方面具有优势，可能有助于BG的血管生成，并进一步增强骨缺损的修复。因此，我们联合应用BG和PBM，并初步研究了它们在体内和体外对骨再生的累加效应。结果显示，BG与PBM联合应用显著增强了人骨髓间充质干细胞的增殖、成骨相关基因的表达及矿化，这比单独应用BG或PBM更好。在体内研究中，组织学染色结果表明，BG在术后6周诱导缺损内部形成新骨，并促进新骨重建。显微计算机断层扫描结果进一步证实，BG与PBM联合应用加速了骨形成和成熟，提高了骨再生的速度和质量，并促进了骨修复。总之，在最佳的BG和PBM参数下，BG与PBM联合应用在促进骨再生方面产生了累加效应。
