Do Kyung-Hyo, Seo Kwangwon, Jung Myunghwan, Lee Woo-Kon, Lee Wan-Kyu
College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of Korea.
Department of Microbiology, College of Medicine, Gyeongsang National University, Jinju 52727, Republic of Korea.
Animals (Basel). 2023 Mar 24;13(7):1154. doi: 10.3390/ani13071154.
The aim of this study was to compare the virulence factors and antimicrobial resistance of the most common pathogenic strains in swine and patients with diarrhea in Korea. We examined virulence genes and antimicrobial susceptibility in 85 and 61 strains isolated from swine and patients with diarrhea, respectively. The most prevalent pathogen in swine was enterotoxigenic (ETEC) (47.1%), followed by Shiga toxin-producing (STEC) (32.9%). Similarly, the majority of the patient isolates (50.8%) were proven to be STEC, the most common pathotype, followed by ETEC (23.0%). We found that swine isolates had significantly higher resistance than patient isolates, especially to fluoroquinolones (ciprofloxacin: 37.5% and 16.1%; norfloxacin: 29.7% and 16.1%, respectively). Additionally, sequence type (ST) 100 (swine: 21; patients: 4), ST 1 (swine: 21, patients: 2), ST 10 (swine: 8; patients: 6), ST 641 (swine: 3, patients: 2), and ST 88 (swine: 2, patients: 11) were detected in both swine and humans. In addition, we confirmed that isolates from swine and patients had similar virulence traits and were phylogenetically similar. According to these findings, swine and humans are susceptible to cross infection and the transfer of antimicrobial resistance.
本研究的目的是比较韩国猪和腹泻患者中最常见致病菌株的毒力因子和抗菌药物耐药性。我们分别检测了从猪和腹泻患者中分离出的85株和61株菌株的毒力基因和抗菌药物敏感性。猪中最常见的病原体是产肠毒素性大肠杆菌（ETEC）（47.1%），其次是产志贺毒素大肠杆菌（STEC）（32.9%）。同样，大多数患者分离株（50.8%）被证实为STEC，这是最常见的致病型，其次是ETEC（23.0%）。我们发现猪分离株的耐药性显著高于患者分离株，尤其是对氟喹诺酮类药物（环丙沙星：分别为37.5%和16.1%；诺氟沙星：分别为29.7%和16.1%）。此外，在猪和人类中均检测到序列型（ST）100（猪：21株；患者：4株）、ST1（猪：21株；患者：2株）、ST10（猪：8株；患者：6株）、ST641（猪：3株；患者：2株）和ST88（猪：2株；患者：11株）。此外，我们证实来自猪和患者的分离株具有相似的毒力特征，并且在系统发育上相似。根据这些发现，猪和人类易发生交叉感染和抗菌药物耐药性的转移。
