Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju, 28644, Republic of Korea.
BMC Microbiol. 2022 Aug 16;22(1):199. doi: 10.1186/s12866-022-02604-z.
Escherichia (E.) coli causes colibacillosis in swine and humans, and is frequently associated with antimicrobial resistance. In this study we aimed to compare antimicrobial resistance, O-serogroups, virulence genes, and multi-locus sequence type of E. coli between isolates from pigs and patients suffering from diarrhea, and the most prevalent pathogenic E. coli strain from swine isolates in Korea.
We tested 64 and 50 E. coli strains from pigs and patients suffering from diarrhea for antimicrobial susceptibility test, virulence genes, O-serogroups, and multi-locus sequence typing.
We confirmed that isolates from swine showed significantly higher resistance than from those from patients, especially to fluoroquinolone (ciprofloxacin: 37.5 and 10.0%; norfloxacin: 29.7 and 8.0%, respectively). Stx1 (46.0%) was most frequently detected in patients followed by stx2 (38.0%). There was no significant difference in stx2 (swine: 23.4%, patients: 38.0%). In isolates from patients, O157 (12.0%) was the most prevalent O-serogroup, and two isolates (3.1%) from pigs were confirmed to have O157. Additionally, sequence type (ST) 10 (swine: 6 isolates, patients: 2 isolates) and ST 88 (swine: 2 isolates, patients: 1 isolate) were simultaneously detected.
We found that both isolates from swine and human had the stx2 gene, which could cause severe disease. Moreover, antimicrobial resistance was significantly higher in pigs than in patients. These results suggest that pig could act as a reservoir in human infection and antimicrobial resistance could be transferred to human from pigs.
大肠杆菌（E. coli）可引起猪和人类的大肠杆菌病，并且经常与抗微生物药物耐药性有关。在本研究中，我们旨在比较猪源和腹泻患者源大肠杆菌分离株的抗微生物药物耐药性、O 血清群、毒力基因和多位点序列型，并比较韩国猪源分离株中最常见的致病性大肠杆菌菌株。
我们对 64 株猪源和 50 株腹泻患者源大肠杆菌进行了抗微生物药物敏感性试验、毒力基因、O 血清群和多位点序列型检测。
我们证实，猪源分离株的耐药性明显高于患者源分离株，尤其是对氟喹诺酮类药物（环丙沙星：37.5%和 10.0%；诺氟沙星：29.7%和 8.0%）。在患者源分离株中，stx1（46.0%）的检出率最高，其次是 stx2（38.0%）。stx2 在患者源分离株中没有显著差异（猪源：23.4%，患者源：38.0%）。在患者源分离株中，O157（12.0%）是最常见的 O 血清群，而 2 株（3.1%）猪源分离株被证实为 O157 型。此外，同时检测到序列型 10（猪源：6 株，患者源：2 株）和 ST88（猪源：2 株，患者源：1 株）。
我们发现猪源和人源分离株均携带可引起严重疾病的 stx2 基因。此外，猪源分离株的抗微生物药物耐药性明显高于患者源分离株。这些结果表明，猪可能成为人类感染的储主，并且抗微生物药物耐药性可能从猪转移到人类。
