Zhang Wei, Chen Jiao, Xie Ling
School of Electronic Engineering, Changzhou College of Information Technology, China.
Department of Intelligent Equipment, Changzhou College of Information Technology, China.
Anal Methods. 2025 Jan 30;17(5):882-891. doi: 10.1039/d4ay02088j.
Typical biosensing platforms are based on the "lock-and-key" approach, providing high specificity and sensitivity for environmental and food safety monitoring. However, they are limited in their ability to detect multiple analytes simultaneously. With the use of pattern identification methods, biosensor arrays can detect faint fluctuations caused by multiple analytes with similar properties in complex systems. As a simple and efficient detection tool, optical biosensor arrays have become crucial for on-site and visible environmental and food safety monitoring. To enhance their practical applications, enzyme-like nanomaterial (nanozyme)-based biosensor arrays have been developed and integrated into optical biosensing platforms, leveraging their exposed active sites and tunable catalytic capabilities. For the development of an optical biosensor array, it is essential to incorporate multiple biosensing elements that can specifically interact with analytes to produce distinct "fingerprint" signals, enabling the differentiation of different targets pattern identification. This review provides a comprehensive overview of nanozyme-based optical biosensor arrays for environmental and food safety monitoring. It explores the selective approaches of nanozyme-based colorimetric and fluorescent biosensor arrays, compares detection platforms utilizing nanozyme systems, and emphasizes the application of nanozyme-based optical biosensor arrays for environmental and food hazard monitoring. By evaluating current trends and summarizing both prospects and challenges, this review offers valuable guidance for the rational design of unique nanozyme-based optical biosensor arrays.
典型的生物传感平台基于“锁钥”方法，在环境和食品安全监测方面具有高特异性和灵敏度。然而，它们在同时检测多种分析物的能力上存在局限。通过使用模式识别方法，生物传感器阵列能够检测复杂系统中由多种具有相似性质的分析物引起的微弱波动。作为一种简单高效的检测工具，光学生物传感器阵列对于现场和可视化的环境及食品安全监测至关重要。为了增强其实际应用，基于类酶纳米材料（纳米酶）的生物传感器阵列已被开发并集成到光学生物传感平台中，利用其暴露的活性位点和可调的催化能力。对于光学生物传感器阵列的开发，纳入多个能够与分析物特异性相互作用以产生独特“指纹”信号的生物传感元件至关重要，这能够通过模式识别区分不同目标。本综述全面概述了用于环境和食品安全监测的基于纳米酶的光学生物传感器阵列。它探讨了基于纳米酶的比色和荧光生物传感器阵列的选择性方法，比较了利用纳米酶系统的检测平台，并强调了基于纳米酶的光学生物传感器阵列在环境和食品危害监测中的应用。通过评估当前趋势并总结前景与挑战，本综述为合理设计独特的基于纳米酶的光学生物传感器阵列提供了有价值的指导。
