Jang Bian, Amirshaghaghi Ahmad, Choi Jeongmoon, Miller Joann, Issadore David A, Busch Theresa M, Cheng Zhiliang, Tsourkas Andrew
Department of Bioengineering, University of Pennsylvania, 210 S. 33rd Street, 435 Skirkanich Hall, Philadelphia, Pennsylvania 19104, United States.
Department of Chemical and Biomolecular Engineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States.
ACS Nano. 2025 Jan 14;19(1):1794-1808. doi: 10.1021/acsnano.4c16600. Epub 2025 Jan 3.
Nanoparticles have gained attention as drug delivery vehicles for cancer treatment, but often struggle with poor tumor accumulation and penetration. Single external magnets can enhance magnetic nanoparticle delivery but are limited to superficial tumors due to the rapid decline in the magnetic field strength with distance. We previously showed that a 2-magnet device could extend targeting to greater tissue depths. Here, we improve on this approach by constructing an 8-magnet device arranged in an annular Halbach array, which facilitates radial outward movement of magnetic nanoparticles from the bore's center. Using chlorin e6-coated magnetic nanoclusters (Ce6 clusters) with densely packed cobalt-doped superparamagnetic iron oxide nanoparticles, we demonstrated nearly a 7-fold improvement in nanoparticle movement through a porous matrix compared to the 2-magnet approach. This resulted in enhanced magnetic resonance contrast, accumulation, and penetration of Ce6 clusters into 4T1 triple-negative breast tumors in mice, leading to improved photodynamic therapy and highlighting the potential therapeutic application of the 8-magnet device.
纳米颗粒作为癌症治疗的药物递送载体已受到关注，但常常在肿瘤蓄积和渗透能力差方面存在问题。单个外部磁体可增强磁性纳米颗粒的递送，但由于磁场强度随距离迅速下降，其仅限于浅表肿瘤。我们之前表明，一种双磁体装置可将靶向范围扩展到更深的组织深度。在此，我们通过构建一个排列成环形哈尔巴赫阵列的八磁体装置改进了这种方法，该装置有助于磁性纳米颗粒从孔的中心径向向外移动。使用包覆有二氢卟吩e6的磁性纳米团簇（Ce6团簇）以及密集堆积的钴掺杂超顺磁性氧化铁纳米颗粒，我们证明与双磁体方法相比，纳米颗粒通过多孔基质的移动能力提高了近7倍。这导致Ce6团簇在小鼠4T1三阴性乳腺肿瘤中的磁共振对比度增强、蓄积增加且渗透增强，从而改善了光动力疗法，并突出了八磁体装置的潜在治疗应用。
