Lee Sangsu, Ahn Chorong, Kim Ye-Jin, Choi Seong-Min, Ko Jung-Woo, Kim Changmu, Park Cheon-Seok
Department of Food Science and Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea.
Species Diversity Research Division, National Institute of Biological Resources, Incheon 22689, Republic of Korea.
Int J Syst Evol Microbiol. 2025 Jan;75(1). doi: 10.1099/ijsem.0.006622.
A novel yeast species, isolated from the bark of pine trees in Gyeongju, South Korea, and designated as KCTC 37304 (ex-type KACC 410729), is characterized by its genetic, morphological and physiological properties. Molecular phylogenetic analysis involving the D1/D2 domain of the 26S LSU rRNA gene and the internal transcribed spacer (ITS) region confirms that it belongs to the genus . In comparison to CBS:10065, the type strain of its closest relative, KCTC 37304 exhibits 8 nucleotide substitutions (2.07%) in the D1/D2 domain and 54 substitutions (8.47%) in the ITS region. Morphologically, cells of KCTC 37304 are globose to ovoid, which distinguishes them from the cylindrical cells of . Physiologically, KCTC 37304 does not assimilate methanol or ethanol but can utilize succinate and xylitol, further differentiating it from . Based on these distinctive genetic, morphological and physiological features, we propose the new species sp. nov., with KCTC 37304 (ex-type strain KACC 410729) designated as the holotype.
从韩国庆州松树树皮中分离出的一种新型酵母菌种，命名为KCTC 37304（模式菌株KACC 410729），具有独特的遗传、形态和生理特性。通过对26S LSU rRNA基因的D1/D2结构域和内部转录间隔区（ITS）进行分子系统发育分析，证实它属于 属。与最相近的模式菌株CBS:10065相比，KCTC 37304在D1/D2结构域有8个核苷酸替换（约2.07%），在ITS区域有54个替换（约8.47%）。形态上，KCTC 37304的细胞呈球形至卵形，这使其与 属的圆柱形细胞有所区别。生理上，KCTC 37304不能同化甲醇或乙醇，但能利用琥珀酸和木糖醇，这进一步将其与 属区分开来。基于这些独特的遗传、形态和生理特征，我们提出新物种 sp. nov.，将KCTC 37304（模式菌株KACC 410729）指定为模式种。
