Hsieh Chen-Che, Hou Chih-Yao, Lei Hsiao-Yun, Khumsupan Darin, Chai Huey-Jine, Lim Pek-Kui, Hsu Cheng-Chih, Wu Sz-Jie, Cheng Kai-Wen, Chen Yi-Chen, Cheng Kuan-Chen
Institute of Food Science Technology, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Taipei, Taiwan, R.O.C.
Department of Seafood Science, College of Hydrosphere, National Kaohsiung University of Science and Technology, Kaohsiung 81157, Taiwan, R.O.C.
J Food Drug Anal. 2024 Dec 15;32(4):532-543. doi: 10.38212/2224-6614.3509.
This study reveals the anti-tyrosinase activity of Ganoderma formosanum extracts, pinpointing compounds including gluconic acid, mesalamine, L-pyroglutamic acid, esculetin, 5-hydroxyindole, and salicylic acid, as effective melanin production inhibitors in melanoma cells and zebrafish embryos. Furthermore, multiple molecular docking simulations provided insights into interactions between the identified compounds and tyrosinase, increasing binding affinity up to -16.36 kcal/mol. The enhanced binding of identified compounds to tyrosinase facilitated synergistic inhibitory effects on melanin production. This study highlights the potential of GFE-EA as a source of natural tyrosinase inhibitors and contributes to understanding the role of active compounds extracted from G. formosanum.
本研究揭示了台湾灵芝提取物的抗酪氨酸酶活性，确定了包括葡萄糖酸、美沙拉嗪、L-焦谷氨酸、七叶亭、5-羟基吲哚和水杨酸在内的化合物，它们是黑色素瘤细胞和斑马鱼胚胎中有效的黑色素生成抑制剂。此外，多个分子对接模拟深入了解了所鉴定化合物与酪氨酸酶之间的相互作用，将结合亲和力提高至-16.36千卡/摩尔。所鉴定化合物与酪氨酸酶的结合增强，促进了对黑色素生成的协同抑制作用。本研究突出了台湾灵芝乙醇提取物作为天然酪氨酸酶抑制剂来源的潜力，并有助于理解从台湾灵芝中提取的活性化合物的作用。
