Hashem Amr H, Abdel-Maksoud Mostafa A, Fatima Sabiha, Almutairi Saeedah M, Ghorab Mohamed A, El-Batal Ahmed I, El-Sayyad Gharieb S
Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Nasr City, 11884, Cairo, Egypt.
Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh, 11451, Saudi Arabia.
Sci Rep. 2025 Jan 3;15(1):689. doi: 10.1038/s41598-024-76446-2.
Cancer and microbial infections place a significant burden on the world's health systems and can increase the rate of disease and mortality. In the current study, a novel nanocomposite based on Gum Arabic, silver and copper oxide nanoparticles (GA@Ag-CuO nanocomposite) was synthesized to overcome the problem of microbial infection and in cancer treatment. Characterization using UV-Vis. spectrophotometer reveals that, the observed peak in the spectrum was formed by the observed O.D. at 0.755, and confirmed that the produced GA@Ag-CuO nanocomposite was small and discernible at 360 nm. The particles' diameters varied from 9.5 nm to 49.5 nm, with a mean diameter of 25.53 ± 1.4 nm. The created Gum Arabic filtrate was rich in active functional groups, and the provided polydisperse NPs were intended to reduce, stabilize, and the produced filtrate act as capping agents. Based on the XRD data, the synthesized GA@Ag-CuO nanocomposite was crystallized and had a face-centered (fcc) crystal structure. Biosafety of GA@Ag-CuO nanocomposite was assessed toward Wi 38 normal cell line, where it showed safety toward the tested cell line where IC50 was 154.2 µg/mL. Antimicrobial results confirmed that, GA@Ag-CuO nanocomposite has antibacterial activity with MICs 15.6, 125, 31.25 and 125 µg/mL against S. epidermis, S. aureus, L. plantrum, and S. typhimurium, respectively. Likewise, it showed antifungal activity toward C. albicans and C. neoformans with MICs 62.5 and 15.62 µg/ml, respectively. Moreover, GA@Ag-CuO nanocomposite displayed promising anticancer activity with IC 26.11 and 59.5 µg/ml toward MCF-7 and Hep-G2, respectively. In conclusion, the novel GA@Ag-CuO nanocomposite demonstrated promising antibacterial, antifungal, and anticancer activities.
癌症和微生物感染给全球卫生系统带来了沉重负担，会提高疾病发生率和死亡率。在本研究中，合成了一种基于阿拉伯胶、银和氧化铜纳米颗粒的新型纳米复合材料（GA@Ag-CuO纳米复合材料），以解决微生物感染问题并用于癌症治疗。通过紫外可见分光光度计进行表征，结果显示，光谱中观察到的峰值是由在0.755处观察到的光密度形成的，并证实所制备的GA@Ag-CuO纳米复合材料尺寸较小，在360nm处可分辨。颗粒直径从9.5nm到49.5nm不等，平均直径为25.53±1.4nm。所制备的阿拉伯胶滤液富含活性官能团，所提供的多分散纳米颗粒旨在起到还原、稳定作用，且所制备的滤液充当封端剂。基于X射线衍射数据，所合成的GA@Ag-CuO纳米复合材料呈结晶态，具有面心立方（fcc）晶体结构。对GA@Ag-CuO纳米复合材料针对Wi 38正常细胞系的生物安全性进行了评估，结果表明其对受试细胞系具有安全性，半数抑制浓度（IC50）为154.2μg/mL。抗菌结果证实，GA@Ag-CuO纳米复合材料具有抗菌活性，对表皮葡萄球菌、金黄色葡萄球菌、植物乳杆菌和鼠伤寒沙门氏菌的最低抑菌浓度（MIC）分别为15.6、125、31.25和125μg/mL。同样，它对白色念珠菌和新型隐球菌也显示出抗真菌活性，MIC分别为62.5和15.62μg/ml。此外，GA@Ag-CuO纳米复合材料对MCF-7和Hep-G2分别显示出有前景的抗癌活性，IC分别为26.11和59.5μg/ml。总之，新型GA@Ag-CuO纳米复合材料显示出有前景的抗菌、抗真菌和抗癌活性。
