Anjum Sumreen, Sarwar Mubeen, Mukhtar Adnan, Hussain Tanveer, Liaquat Mehwish, Batool Alia, Alam Muhammad Waqar, Ali Qurban, Ashraf M Irfan
Institutes of Botany, Faculty of Life Sciences, University of the Punjab, Lahore, Pakistan.
Department of Horticulture, University of the Punjab, Lahore, Pakistan.
Sci Rep. 2025 Jan 3;15(1):707. doi: 10.1038/s41598-024-84648-x.
This study investigates the effects of lead stress on tomato plants and explores the potential role of plant growth-promoting rhizobacteria (PGPR) to alleviate this stress. The experiment was conducted in pots, introducing varying lead levels (0, 100, 200, 300, 400, and 500 mg kg⁻¹) using lead nitrate. For rhizobacterial inoculation, pre-characterized LTPGP strains S5 Pseudomonas fluorescens A506 and S10 Pseudomonas fluorescens LMG 2189 were used. Data were recorded on growth parameters (shoot and root length, fresh and dry weight), physiological attributes (chlorophyll content, proline content, electrolyte leakage, and lead accumulation), and antioxidant activities (catalase, peroxidase, superoxide dismutase) in tomato plants. Results revealed that lead contamination significantly impaired plant growth, reducing shoot and root lengths by approximately 49.78-57.62%, and negatively impacted physiological attributes and antioxidant enzyme activities. However, inoculation with PGPR strains S5 and S10 led to notable improvements in growth parameters, enhanced physiological traits, and better antioxidant activities, effectively mitigating lead stress. These findings highlights the potential of PGPR to improve tomato plant resilience to lead contamination.
本研究调查了铅胁迫对番茄植株的影响，并探讨了促生根际细菌（PGPR）缓解这种胁迫的潜在作用。实验在花盆中进行，使用硝酸铅引入不同铅水平（0、100、200、300、400和500 mg kg⁻¹）。对于根际细菌接种，使用预先鉴定的LTPGP菌株荧光假单胞菌A506 S5和荧光假单胞菌LMG 2189 S10。记录了番茄植株的生长参数（地上部和根长、鲜重和干重）、生理特性（叶绿素含量、脯氨酸含量、电解质渗漏和铅积累）以及抗氧化活性（过氧化氢酶、过氧化物酶、超氧化物歧化酶）的数据。结果表明，铅污染显著损害了植株生长，使地上部和根长减少了约49.78 - 57.62%，并对生理特性和抗氧化酶活性产生了负面影响。然而，接种PGPR菌株S5和S10导致生长参数显著改善、生理性状增强以及抗氧化活性提高，有效缓解了铅胁迫。这些发现突出了PGPR提高番茄植株对铅污染恢复力的潜力。
