Lee Hyosun, Chaudhary Dhiraj Kumar, Lee Ki-Eun, Cha In-Tae, Chi Won-Jae, Park Sunho, Seo Taegun, Kim Dong-Uk
Department of Biological Science, College of Science and Engineering, Sangji University, Wonju, 26339, Republic of Korea.
Department of Microbiology, Pukyong National University, Busan, 48513, Republic of Korea.
Curr Microbiol. 2024 Dec 28;82(1):61. doi: 10.1007/s00284-024-04043-7.
During the study of microbial diversity of forest soil in the Republic of Korea, a yellow pigment-producing, Gram-stain-negative, rod-shaped, motile bacterium was isolated and designated as strain 1W2. This strain grew at temperature of 10-37 °C, at pH of 5.0-9.0, and at NaCl concentration of 0-3.0% (w/v). The 16S rRNA gene sequencing and genome sequencing revealed that strain 1W2 is a member of the family Phyllobacteriaceae but exhibits low similarity with known genera, suggesting that this strain is a new genus within the family. This strain showed the closest similarity to the genera Mesorhizobium (96.6-96.9%), Aminobacter (96.4 -96.6%), Aquamicrobium (96.5-96.7%), and Pseudaminobacter (96.6-96.7%). The nearest relative of 1W2 was Mesorhizobium shangrilense CCBAU 65327 with the 16S rRNA gene sequence similarity of 96.9%. The genome size was 5,545,526 bp with DNA G + C content of 64.7%. The values of overall genomic relatedness indices between strain 1W2 and the reference members were 20.4-21.3% for digital DNA-DNA hybridization, 74.0-76.6% for average nucleotide identity, and 68.1-61.2% for amino acids identity. Chemotaxonomic profiling revealed that Q-10 was the sole ubiquinone; summed feature 8 (Cω7c and/or Cω6c), iso-C, and C cyclo ω8c were the predominant fatty acids; and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylcholine, and phosphatidylethanolamine were the major polar lipids. Based on these data, strain 1W2 represents a novel species of a new genus in the family Phyllobacteriaceae. Accordingly, we proposed the name Arvimicrobium flavum gen. nov., sp. nov., with the type strain 1W2 (= KCTC 92441 = NBRC 116019).
在对韩国森林土壤微生物多样性的研究过程中，分离出了一株产黄色色素、革兰氏染色阴性、杆状、具运动性的细菌，命名为1W2菌株。该菌株在10 - 37°C的温度、5.0 - 9.0的pH值以及0 - 3.0%（w/v）的NaCl浓度下生长。16S rRNA基因测序和基因组测序表明，1W2菌株属于叶杆菌科，但与已知属的相似性较低，这表明该菌株是该科内的一个新属。该菌株与中生根瘤菌属（96.6 - 96.9%）、氨基杆菌属（96.4 - 96.6%）、水生微生物属（96.5 - 96.7%）和假氨基杆菌属（96.6 - 96.7%）的相似性最高。1W2的最相近亲缘种是香格里拉中生根瘤菌CCBAU 65327，其16S rRNA基因序列相似性为96.9%。基因组大小为5,545,526 bp，DNA G + C含量为64.7%。1W2菌株与参考菌株之间的全基因组相关性指数，数字DNA - DNA杂交为20.4 - 21.3%，平均核苷酸同一性为74.0 - 76.6%，氨基酸同一性为68.1 - 61.2%。化学分类分析表明，泛醌Q - 10是唯一的泛醌；特征性总和8（Cω7c和/或Cω6c）、异C和C环ω8c是主要脂肪酸；双磷脂酰甘油、磷脂酰甘油、磷脂酰甲基乙醇胺、磷脂酰胆碱和磷脂酰乙醇胺是主要极性脂质。基于这些数据，1W2菌株代表叶杆菌科一个新属的一个新物种。因此，我们提出了新属名Arvimicrobium flavum gen. nov., sp. nov.，模式菌株为1W2（= KCTC 92441 = NBRC 116019）。
