Savastano Maria Cristina, Giannuzzi Federico, Savastano Alfonso, Cestrone Valentina, Boselli Francesco, Carlà Matteo Mario, D'Onofrio Nicola Claudio, Biagini Ilaria, Rizzo Clara, Bianchi Maria, Valentini Caterina Giovanna, Teofili Luciana, Urbani Andrea, Iavarone Federica, Rizzo Stanislao
Ophthalmology Unit, Fondazione Policlinico Universitario A. Gemelli IRCCS, Largo A. Gemelli, 1, 00168, Rome, Italy.
Università Cattolica del Sacro Cuore, Rome, Italy.
Clin Proteomics. 2025 Jan 3;22(1):1. doi: 10.1186/s12014-024-09524-2.
Our objective is to determine the protein and complements constituents of Cord blood Platelet-rich plasma (CB-PRP), based on the hypothesis that it contains beneficial components capable of arresting or potentially decelerating the advancement of atrophic age-related macular degeneration (dry-AMD), with the support of radiomics. Two distinct pools of CB-PRP were assessed, each pool obtained from a total of 15 umbilical cord-blood donors. One aliquot of each pool respectively was subjected to proteomic analysis in order to enhance the significance of our findings, by identifying proteins that are shared between the two sample pools and gaining insights into the pathways they are associated with. The bioinformatics analysis was developed using Reactome software. Three-hundred-seven (307) distinct proteins were found. Two hundred fifteen (215) of the elements mentioned above are shared by both pools. Seventy (70) elements are exclusive to pool S1, while pool S2 contains 22. We detected 109 representative and statistically significant pathways out of 549. We found proteins related to the immune system, signal transduction, vesicle-mediated transport, cell-cell communication, hemostasis, cellular responses to stimuli, cell cycle, and developmental biology. The analysis showed the presence of P15692-12, representing VEGF factor A, long form. With over 200 proteins, the CB-PRP can increase the immune response, including BCR, CD-22, FCGR, phospholipids, IL-10, FCGR-3A, and others. Discovering crucial trophic and complement-regulating variables is highly significant for potential applications in dry AMD. Our future research will examine the effects of intravitreal CB-PRP on dry-AMD eyes.
我们的目标是确定脐带血富含血小板血浆（CB-PRP）的蛋白质和补体成分，基于这样的假设，即在放射组学的支持下，它含有能够阻止或潜在延缓萎缩性年龄相关性黄斑变性（干性AMD）进展的有益成分。对两个不同的CB-PRP样本池进行了评估，每个样本池共取自15名脐带血捐献者。为了提高我们研究结果的显著性，每个样本池分别取一份进行蛋白质组分析，通过识别两个样本池之间共有的蛋白质，并深入了解它们所关联的途径。使用Reactome软件进行生物信息学分析。共发现307种不同的蛋白质。上述元素中有215种在两个样本池中都有。70种元素仅存在于样本池S1中，而样本池S2含有22种。在549条通路中，我们检测到109条具有代表性且具有统计学意义的通路。我们发现了与免疫系统、信号转导、囊泡介导的运输、细胞间通讯、止血、细胞对刺激的反应、细胞周期和发育生物学相关的蛋白质。分析显示存在代表VEGF因子A长形式的P15692-12。CB-PRP含有200多种蛋白质，可增强免疫反应，包括BCR、CD-22、FCGR、磷脂、IL-10、FCGR-3A等。发现关键的营养和补体调节变量对于干性AMD的潜在应用具有高度重要性。我们未来的研究将考察玻璃体内注射CB-PRP对干性AMD眼睛的影响。
