Fan Guoqiang, Chen Wenjing, He Jianxing, Wang Danping, Yang Xiaojing
Key Laboratory of Animal Physiology & Biochemistry, Nanjing Agricultural University, Nanjing 210095, PR China.
Key Laboratory of Animal Physiology & Biochemistry, Nanjing Agricultural University, Nanjing 210095, PR China; MOE Joint International Research Laboratory of Animal Health and Food Safety, Nanjing Agricultural University, Nanjing 210095, PR China.
Res Vet Sci. 2025 Mar;184:105526. doi: 10.1016/j.rvsc.2024.105526. Epub 2024 Dec 31.
Previous research has identified bile acids (BAs) as a valuable supplement for animal feed, especially in the poultry industry. However, there is limited research on the use of bile acids as a preventative measure against intestinal inflammation in broilers. This study aims to investigate the impact of dietary BAs on LPS-triggered intestinal inflammation in broilers. 180 Arbor Acres broilers were randomly divided into four group: (1) broilers receiving a standard diet (Con group); (2) broilers from the Con category subjected to LPS challenge (LPS group); (3) broilers on a diet supplemented with BAs compound and exposed to LPS (BA+LPS group); and (4) broilers on a diet enriched with lithocholic acid (LCA) and challenged with LPS (LCA + LPS group).The results showed that the LPS challenge caused a notable rise in liver mass, plasma AST concentrations, and levels of inflammatory cytokines (P < 0.05). BAs compounds or LCA improved intestinal morphological damage, inflammation response and bile acid metabolism (P < 0.05). Furthermore, analysis of 16S rRNA gene sequences revealed that supplementation with BAs compounds or LCA mitigated the reduction in bacterial diversity, while also increasing the abundance of operational taxonomic units (OTUs) associated with Bacteroides and Bifidobacterium. Additionally, the increased abundance of Candidatus_Arthromitus due to BAs compound or LCA supplementation showed a significant negative correlation with the concentrations of intestinal inflammatory cytokines (P < 0.05). These results suggest that the supplementation of BAs compound or LCA has the potential to alleviate intestinal inflammation and regulate gut microbiota in broilers subjected to LPS challenge.
先前的研究已确定胆汁酸（BAs）是动物饲料的一种有价值的添加剂，尤其是在禽类养殖业中。然而，关于将胆汁酸用作预防肉鸡肠道炎症措施的研究有限。本研究旨在探讨日粮胆汁酸对脂多糖（LPS）引发的肉鸡肠道炎症的影响。180只艾维茵肉鸡被随机分为四组：（1）接受标准日粮的肉鸡（对照组）；（2）来自对照组且受到LPS攻击的肉鸡（LPS组）；（3）日粮添加胆汁酸化合物并暴露于LPS的肉鸡（BA + LPS组）；以及（4）日粮富含石胆酸（LCA）并受到LPS攻击的肉鸡（LCA + LPS组）。结果表明，LPS攻击导致肝脏重量、血浆天冬氨酸转氨酶（AST）浓度和炎性细胞因子水平显著升高（P < 0.05）。胆汁酸化合物或LCA改善了肠道形态损伤、炎症反应和胆汁酸代谢（P < 0.05）。此外，16S rRNA基因序列分析显示，添加胆汁酸化合物或LCA减轻了细菌多样性的降低，同时还增加了与拟杆菌属和双歧杆菌属相关的可操作分类单元（OTU）的丰度。此外，由于添加胆汁酸化合物或LCA导致的类芽孢杆菌丰度增加与肠道炎性细胞因子浓度呈显著负相关（P < 0.05）。这些结果表明，添加胆汁酸化合物或LCA有潜力减轻受到LPS攻击的肉鸡的肠道炎症并调节肠道微生物群。
