Ovis-Sánchez Julián O, Vital-Jácome Miguel, Buitrón Germán, Cervantes-Avilés Pabel, Carrillo-Reyes Julián
Laboratorio de Investigación en Procesos Avanzados de Tratamiento de Aguas, Unidad Académica Juriquilla, Instituto de Ingeniería, Universidad Nacional Autónoma de México, Querétaro 76230, México.
Escuela de Ingeniería y Ciencias, Tecnológico de Monterrey, Campus Puebla, Vía Atlixcáyotl 5718, Reserva Territorial Atlixcáyotl, Puebla, 72453 Puebla, México.
Bioresour Technol. 2025 Mar;419:132037. doi: 10.1016/j.biortech.2025.132037. Epub 2025 Jan 3.
Microalgae-bacteria-based systems are an emerging and promising approach for wastewater treatment plants (WWTP), having nutrient and antibiotic resistance removal comparable to conventional technologies. Still, antibiotic-resistance genes and bacteria (ARG and ARB) can proliferate in microalga-bacteria aggregates (MABA), a concern to control. Different temperature regimes of MABA continuous anaerobic digestion (AD), thermophilic (55 °C), and mesophilic (35 °C) were evaluated in this study as a strategy to eliminate ARB and ARGs. Plate counting techniques and metagenomic-based analysis revealed that thermophilic temperature had a better performance, achieving ARB log reductions of 1.1 to 1.7 for various antibiotics and significantly reduced ARG abundance up to 19.5 ± 0.8 ppm. The microbiome selection, the mobilome restriction, and directed functionality to thermal stress resistance were the main mechanisms responsible for resistome reduction at thermophilic conditions. Thermophilic AD effectively manages antibiotic resistance in microalgae-bacteria aggregates, which has important implications for wastewater treatment and reduces environmental risks.
基于微藻-细菌的系统是污水处理厂一种新兴且有前景的方法，其在去除营养物质和抗生素抗性方面可与传统技术相媲美。尽管如此，抗生素抗性基因和细菌（ARG和ARB）仍可能在微藻-细菌聚集体（MABA）中增殖，这是一个需要控制的问题。本研究评估了MABA连续厌氧消化（AD）在不同温度条件下，即嗜热（55°C）和中温（35°C），作为消除ARB和ARG的策略。平板计数技术和基于宏基因组的分析表明，嗜热温度表现更佳，对各种抗生素实现了1.1至1.7的ARB对数减少，并将ARG丰度显著降低至19.5±0.8 ppm。微生物群落选择、可移动基因组限制以及对热应激抗性的定向功能是嗜热条件下抗性组减少的主要机制。嗜热AD有效地管理了微藻-细菌聚集体中的抗生素抗性，这对废水处理具有重要意义，并降低了环境风险。
