Xu Fangfang, Zhao Guangbin, Gong Yuxin, Liang Xiang, Yu Ming, Cui Hao, Xie Linyang, Zhu Nan, Zhu Xuan, Shao Xiaoxi, Qi Kun, Lu Bingheng, Tu Junbo, Na Sijia
Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi' an Jiaotong University, Xi' an, 710004, China.
Clinical Research Center of Shaanxi Province for Dental and Maxillofacial Diseases, College of Stomatology, Xi'an Jiaotong University, Xi' an, 710004, China.
Adv Healthc Mater. 2025 Mar;14(6):e2403388. doi: 10.1002/adhm.202403388. Epub 2025 Jan 5.
The regulation of the charged microenvironment around implants is an effective way to promote osseointegration. Although homeostasis of the charged microenvironment plays an integral role in tissues, current research is externally invasive and unsuitable for clinical applications. In this study, functional materials with different surface potential differences are prepared by changing the spatial layout of Ta and Ag on the surface of a Ti-6Al-4V alloy (TC4). This naturally formed an endogenous electric field (EEF) with a negatively charged cell membrane after in vivo implantation and promoted osseointegration at the interface between the bone and implant through the upregulation of Ca concentration and activation of subsequent pathways. Interestingly, the promotion of stem cell differentiation, regulation of the direction of immune cell polarization, and antibacterial efficacy are determined by the free charge contained in the implant, rather than by the magnitude of the surface potential difference. This functional implant represents a unique strategy for regulating the charged microenvironment around the implant and enhancing osseointegration, thereby providing ideas and technical approaches for the clinical development of novel implant materials.
调节植入物周围的带电微环境是促进骨整合的有效方法。尽管带电微环境的稳态在组织中起着不可或缺的作用，但目前的研究具有外部侵入性，不适合临床应用。在本研究中，通过改变Ti-6Al-4V合金（TC4）表面Ta和Ag的空间布局，制备了具有不同表面电位差的功能材料。体内植入后，这自然形成了一个与带负电细胞膜的内源性电场（EEF），并通过钙浓度的上调和后续途径的激活促进了骨与植入物界面处的骨整合。有趣的是，干细胞分化的促进、免疫细胞极化方向的调节和抗菌效果取决于植入物中所含的自由电荷，而不是表面电位差的大小。这种功能性植入物代表了一种调节植入物周围带电微环境和增强骨整合的独特策略，从而为新型植入材料的临床开发提供思路和技术途径。
